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生物源
mouse
品質等級
共軛
ALEXA FLUOR™ 488
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
C4, monoclonal
物種活性
human
物種活性（以同源性預測）
chicken (based on 100% sequence homology)
製造商／商標名
Chemicon®
技術
immunofluorescence: suitable
同型
IgG2bκ
NCBI登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... ACTA1(58)
一般說明
Actins are ubiquitous eukaryotic proteins that serve as a multi-functional, basic building blocks of cytoskeletal microfilaments. They play critical roles in a wide range of cellular processes, including cell division, cell migration, chromatin remodeling, trascriptional regulation and vesicle trafficking. These funstions are attributed to their ability to form filaments, which can quickly assemble and disassemble depending upon the needs of the cell. At least six different actin types have been reported in mammals. Although actins show about 90% overall sequence homology, isoforms do not show spatial, temporal and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. Beta and gamma-actins, also known as cytoplasmic actins, are highly conserved in higher animals and are predominantly expressed in non-muscle cells where they control cell structure. Exocytosis, and motility. They are nearly identical proteins and differ only in four amino acids at the N-terminal region. The other four actin isoforms are typically found in specific adult muscle tissue types. Alpha-cardiac and alpha-skeletal actins are expressed in striated cardiac and skeletal muscles, respectively. Alpha and gamma actins are primarily found in vascular smooth muscle and enteric smooth muscles, respectively. It has been shown that under calcium-bound conditions, beta-actin exhibits more dynamic behavior than gamma-actin with higher rates of polymerization and depolymerization. Also, beta- and gamma-actins can readily copolymerize, and the resulting filaments exhibit polymerization and depolymerization rates that vary depending on the ration of beta- to gamma-actin (Lessard, JL.,et al.(1988). Cell Motility Cytoskeleton 10(3); 349-362.
特異性
MAB1501X is a pan-actin antibody that binds to an epitope in a highly conserved region of actin; therefore, this antibody reacts with all six isoforms of vertabrate actin (Lessard, 1988). Reacts with both globular (G) and fillimentous (F) forms of actin and does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. However, this antibody does increase the extent of polymerization when used at a lower ratio of antibody to actin. In addition to labeling myotubes, anti-actin stains myoblasts and fibroblasts. Although clone C4 is prepared as an antibody to chicken gizzard muscles actin, it reacts with actins from all vertebrates, as well as with Dictyostelium discoideum and Physarum polycephalum actins (Lessard, 1988).
Other species have not been tested.
免疫原
Epitope is conserved in all known actins.
Purified chicken gizzard actin (Lessard, 1988).
應用
Anti-Actin Antibody, clone C4, Alexa Fluor 488 conjugated detects level of Actin & has been published & validated for use in IF.
Immunofluorescence: 1:200-1:1000
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
品質
Evaluatied on a representative lot by immunofluorescence using HeLa cells.
標靶描述
~43 kDa
聯結
Replaces: MAB1501X
外觀
Protein G Purified
Purified in PBS with 15 mg/mL BSA and 0.05% NaN3, conjugated to Alexa Fluor 488.
儲存和穩定性
Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.
分析報告
Control
HeLa cells
HeLa cells
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
ALEXA FLUOR is a trademark of Life Technologies
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Alexa Fluor™ is a registered trademark of Molecular Probes, Inc.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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