跳转至内容
Merck
所有图片(2)

Key Documents

HMYOMAG-56K

Millipore

MILLIPLEX® 人肌细胞因子磁珠板

The Human Myokine Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 15 myokine protein biomarkers in human serum, plasma and cell/tissue culture samples.

登录查看公司和协议定价


About This Item

分類程式碼代碼:
12161503
eCl@ss:
32161000
NACRES:
NA.84

品質等級

物種活性

human

製造商/商標名

Milliplex®

assay range

accuracy: 100%
(Apelin)

accuracy: 92%
(IL-15)

accuracy: 96%
(FSTL-1)

accuracy: 97%
(LIF)

accuracy: 98%
(Oncostatin M (OSM))

sensitivity: 0.9 pg/mL
(MinDC+2SD; IL-6)

sensitivity: 14 pg/mL
(MinDC+2SD; FGF-21)

sensitivity: 259 pg/mL
(MinDC+2SD; Myostatin/GDF8)

sensitivity: 281 pg/mL
(MinDC+2SD; Irisin)

sensitivity: 4 pg/mL
(MinDC+2SD; LIF)

sensitivity: 40 pg/mL
(MinDC+2SD; Fractalkine/CX3CL1)

sensitivity: 5 pg/mL
(MinDC+2SD; IL-15)

sensitivity: 59 pg/mL
(MinDC+2SD; Osteocrin/Musclin)

sensitivity: 6 pg/mL
(MinDC+2SD; Oncostatin M (OSM))

sensitivity: 64 pg/mL
(MinDC+2SD; Apelin)

sensitivity: 7.5 ng/mL
(MinDC+2SD; Osteonectin (SPARC))

sensitivity: 737 pg/mL
(MinDC+2SD; Follistatin-like Protein 1 (FSTL1))

sensitivity: 815 pg/mL
(MinDC+2SD; Erythropoietin (EPO))

sensitivity: 9 pg/mL
(MinDC+2SD; BDNF)

sensitivity: 9.8 pg/mL
(MinDC+2SD; FABP3)

standard curve range: 0.6-2,400 pg/mL
(IL-6)

standard curve range: 10-40,000 pg/mL
(LIF)

standard curve range: 122-500,000 pg/mL
(Apelin)

standard curve range: 2-10,000 pg/mL
(BDNF)

standard curve range: 2-10,000 pg/mL
(IL-15)

standard curve range: 2-6,000 pg/mL
(Oncostatin M (OSM))

standard curve range: 24-100,000 pg/mL
(FABP3)

standard curve range: 244-1,000,000 pg/mL
(Erythropoietin (EPO))

standard curve range: 244-1,000,000 pg/mL
(Irisin)

standard curve range: 488-2,000,000 pg/mL
(Follistatin-like Protein 1 (FSTL1))

standard curve range: 488-2,000,000 pg/mL
(Myostatin/GDF8)

standard curve range: 49-200,000 pg/mL
(Fractalkine/CX3CL1)

standard curve range: 49-200,000 pg/mL
(Osteocrin/Musclin)

standard curve range: 5-20,000 pg/mL
(FGF-21)

standard curve range: 7-30,000 ng/mL
(Osteonectin (SPARC))

inter-assay cv: <15%
intra-assay cv: <10%
(Apelin)

inter-assay cv: <15%
intra-assay cv: <10%
(BDNF)

inter-assay cv: <15%
intra-assay cv: <10%
(Erythropoietin (EPO))

inter-assay cv: <15%
intra-assay cv: <10%
(FABP3)

inter-assay cv: <15%
intra-assay cv: <10%
(FGF-21)

inter-assay cv: <15%
intra-assay cv: <10%
(Follistatin-like Protein 1 (FSTL1))

inter-assay cv: <15%
intra-assay cv: <10%
(Fractalkine/CX3CL1)

inter-assay cv: <15%
intra-assay cv: <10%
(IL-15)

inter-assay cv: <15%
intra-assay cv: <10%
(IL-6)

inter-assay cv: <15%
intra-assay cv: <10%
(Irisin)

inter-assay cv: <15%
intra-assay cv: <10%
(LIF)

inter-assay cv: <15%
intra-assay cv: <10%
(Myostatin/GDF8)

inter-assay cv: <15%
intra-assay cv: <10%
(Oncostatin M (OSM))

inter-assay cv: <15%
intra-assay cv: <10%
(Osteocrin/Musclin)

inter-assay cv: <20%
intra-assay cv: <10%
(Osteonectin (SPARC))

技術

multiplexing: suitable

檢測方法

fluorometric (Luminex xMAP)

運輸包裝

wet ice

一般說明

身体活动期间的肌肉收缩是肌动蛋白释放的重要激活剂。肌动蛋白不仅以自分泌和/或旁分泌的方式调节骨骼肌的生长,而且还可以作为内分泌激素来介导器官间的串扰。肌动蛋白理论为运动引起的肝脏和脂肪组织代谢变化以及免疫和神经元系统的深刻变化提供了分子机制的解释。

MILLIPLEX®人肌动蛋白面板是一种15重试剂盒,用于同时定量人血清、血浆和细胞/组织培养上清液样品中以下任何或所有分析物:Fractalkine/CX3CL1,BDNF,制瘤素M(OSM),骨连接蛋白(SPARC),LIF,FGF-21,促红细胞生成素(EPO),IL-15,IL-6,Apelin,Myostatin/GDF8,鸢尾素,Osteocrin/Musclin,FABP3和卵泡抑素样蛋白(FSTL 1)。该试剂盒采用96孔板,包含冻干标准混合物、两个内部检测质控品,可最多可测定38份样本,重复两次。

Luminex® xMAP®平台使用磁珠免疫分析格式,以实现理想的速度和灵敏度,同时定量多种分析物,从而显著提高生产力,同时节省宝贵的样本量。

面板类型:代谢

應用

  • 分析物:Apelin(APLN)、脑源性神经营养因子(BDNF)、促红细胞生成素(EPO)、脂肪酸结合蛋白3(FABP3)、成纤维细胞生长因子21(FGF21)、Fractalkine(CX3CL1)、卵泡抑素样1蛋白(FSTL-1)、IL-6、IL-15、鸢尾素、白血病抑制性因子(LIF)、肌肉生长抑制素(MSTN/GDF8)、制瘤素M(OSM)、骨调节素(OSTN/Musclin)、骨连接蛋白(SPARC)
  • 推荐的样品类型:人血清、血浆和细胞/组织培养上清液或裂解液
  • 注释:大量的BDNF储存在循环的血小板中,随后在血小板活化后释放。因此,贫血小板血浆对于确保BDNF循环水平的准确测量至关重要。
  • 推荐的样品稀释度:每孔25 μL 1:2稀释的血清或血浆。细胞/组织培养样品可以在未稀释或适当稀释的对照培养基中运行。
  • 分析运行时间:在2-8℃下过夜(16-18小时)或在室温(20-25℃)下2小时。
  • 研究类别:骨
  • 研究子类别:代谢

特點和優勢

通过在此面板中选择可用的分析物来设计多重试剂盒。

其他說明

请联系技术服务部进行稀释度线性研究。

法律資訊

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

訊號詞

Danger

危險分類

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2

標靶器官

Respiratory Tract

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Kelly Ann Hutchinson et al.
Frontiers in physiology, 10, 1188-1188 (2019-10-28)
It is recommended that women accumulate 150-min of weekly moderate-intensity physical activity (MPA) when pregnant. Engaging in regular physical activity (PA) confers many health benefits to both the mother and the fetus. However, the molecular mechanisms by which these health
Javier Diaz-Castro et al.
Frontiers in nutrition, 8, 761213-761213 (2021-11-09)
Practicing exercise is one of the best strategies to promote well-being and quality of life, however physical activity in schoolchildren and adolescents is developed in an unpredictable, intermittent way and in short periods. There are relatively few intervention studies investigating

相关内容

Myokine analysis with multiplex immunoassays, such as the MILLIPLEX® Human Myokine Panel, allow researchers to simultaneously measure multiple muscle-secreted factors, providing more insights into related diseases.

Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门