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Merck
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Key Documents

HMH3-34K

Millipore

MILLIPLEX® HUMAN METABOLIC HORMONE PANEL V3

别名:

human metabolic hormone immunoassay panel, luminex human metabolic hormone multiplex assay, millipore human metabolism multiplex kit

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.47

物種活性

human

品質等級

製造商/商標名

Milliplex®

技術

multiplexing: suitable

檢測方法

fluorometric (Luminex® xMAP®)

儲存溫度

2-8°C

一般說明

Metabolic syndrome is a cluster of conditions that occur together; it includes increased blood pressure, elevated insulin levels, excess body fat around the waist and abnormal cholesterol levels.Key features of metabolic syndrome include insulin resistance, glucose intolerance, hypertension, dyslipidemia and central obsesity- all of which are risk factors for atherosclerosis, coronary heart disease, type 2 diabetes, kidney disease, and even premature death.Adults with metabolic syndrome show a low-grade inflammation, whose link with obesity may be dysregulated adipocyte production of pro- and anti-inflammatory factors. Consequently, research done in this area covers multi-faceted fields of cytokines, acute phase proteins, diabetes, and obesity related hormones, as well as other cardiovascular disease biomarkers.MILLIPLEX® Human Metabolic Hormone Panel V3 is a 14-plex kit to be used for the simultaneous quantification of any or all of the following analytes in human serum, plasma, tissue/cell lysates, and culture supernatant samples: Amylin Active or Total, C-Peptide, Ghrelin Active, GIP, GLP-1 Active or Total, Glucagon, IL-6, Insulin, Leptin, MCP-1/CCL2, PP, PYY, Secretin and TNFα.Note: Amylin Active and Total cannot be run together in the same assay. GLP-1 Active an GLP-1 Total cannot be run together in the same assay.

特異性

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

應用

The Human Metabolic Hormone Panel V3, using the Luminex® xMAP technology, enables the simultaneous analysis of 14 protein biomarkers in human serum, plasma and tissue/lysate and culture supernatant samples.There are 16 biomarkers to choose from in this kit. However, this is a 14-plex kit since the following analytes cannot be run simultaneously: Amylin Active and Total cannot be run together in the same assay. GLP-1 Active and Total cannot be run together in the same assay.The following analytes require the addition of protease inhibitors to the serum or plasma samples (see sample collection and storage section in the protocol for details): GLP-1 (DPPIV inhibitor); Amylin (protease inhibitor cocktail); Glucagon (Aprotinin); Ghrelin (Active) (serine protease inhibitor). The protocol recommends serum or plasma samples be run neat. Tissue culture samples may be run neat or may require a dilution with an appropriate control medium. For optimal sensitivity, use an overnight (16 - 18 hour) incubation. Each well requires 25 uL of sample, standard or controlAnalytes available:Amylin (Total);C-Peptide;Ghrelin (Active);GIP;GLP-1 (Active);GLP-1 (Total);Glucagon;IL-6;Insulin;Leptin;MCP-1;PP;PYY;TNFα;Secretin;Amylin (Active)

特點和優勢

Design your multiplex kit by choosing available analytes within this panel.

包裝

96-well plate

儲存和穩定性

Recommended storage for kit components is 2 - 8°C.

法律資訊

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

象形圖

Skull and crossbonesEnvironment

訊號詞

Danger

危險分類

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


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相关内容

Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.

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