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一般說明
Chemotaxis, or directed cell movement is a fundamental cellular process, necessary for embryonic development, angiogenesis, wound healing, and immune responses, as well as pathological events associated with inflammation and cancer metastasis [Lauffenburger & Horwitz, 1996]. Prior to cell body translocation or chemotaxis, a cell must first extend a dominant pseudopodium (lamellipodium) in the direction of the chemoattractant gradient [Lauffenburger & Horwitz, 1996; Parent & Devreotes, 1999]. Once a dominant pseudopodium is formed, cell movement can begin in the direction of the gradient. Formation of a directional pseudopodium is tightly controlled by signal transduction mechanisms that temporally and spatially regulate changes in focal adhesions, microtubules, and the actin/myosin cytoskeleton [Ridley et al., 1992; Schmidt et al., 1993; Manes et al., 1999; Nobes & Hall, 1999; Eddy et al., 2000].
The CHEMICON Pseudopodia Purification Kit is a novel system designed to specifically isolate and purify extending pseudopodia proteins from the cell body [Cho & Klemke, 2002]. This Kits provides a quick and efficient system to purify pseudopodia proteins and their responses to various natural or synthetic compounds that specifically impact the microenvironment of the pseudopodial organelle including signaling processes, integrins or other adhesion receptors, and unique gene products. This kit provides a novel method for pseudopodia protein purification; identify signaling complex formation as a result of chemotaxis stimuli.
For Research Use Only; Not for use in diagnostic procedures
The CHEMICON Pseudopodia Purification Kit is a novel system designed to specifically isolate and purify extending pseudopodia proteins from the cell body [Cho & Klemke, 2002]. This Kits provides a quick and efficient system to purify pseudopodia proteins and their responses to various natural or synthetic compounds that specifically impact the microenvironment of the pseudopodial organelle including signaling processes, integrins or other adhesion receptors, and unique gene products. This kit provides a novel method for pseudopodia protein purification; identify signaling complex formation as a result of chemotaxis stimuli.
For Research Use Only; Not for use in diagnostic procedures
應用
Research Category
Cell Structure
Cell Structure
成分
Pseudopodia Purification Inserts - (Catalog No. 71608) 1 X 6 well plate containing 6 X 24mm 3.0-micrometer transwells.
Western Blot Lysis Buffer - (Catalog No. 71606) 20 mL of 50 mM Tris, pH 7.4, 5 mM EDTA, 150 mM NaCl, 1% SDS
Immunoprecipitation Buffer - (Catalog No. 71601) 20 mL of 50 mM Tris, pH 7.4, 5 mM EDTA, 150 mM NaCl, 1% Triton-X-100
Cell Scrapers - (Catalog No.71603) 3 each
Forceps - (Catalog No. 10203) 1 each
Cotton Swabs - (Catalog No. 10202) 14 swabs
Western Blot Lysis Buffer - (Catalog No. 71606) 20 mL of 50 mM Tris, pH 7.4, 5 mM EDTA, 150 mM NaCl, 1% SDS
Immunoprecipitation Buffer - (Catalog No. 71601) 20 mL of 50 mM Tris, pH 7.4, 5 mM EDTA, 150 mM NaCl, 1% Triton-X-100
Cell Scrapers - (Catalog No.71603) 3 each
Forceps - (Catalog No. 10203) 1 each
Cotton Swabs - (Catalog No. 10202) 14 swabs
儲存和穩定性
Store Kit and Materials at 2-8ºC until their expiration date
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
訊號詞
Warning
危險聲明
危險分類
Aquatic Chronic 3 - Eye Irrit. 2
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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