推荐产品
产品名称
Anti-Actin Antibody, smooth muscle, clone ASM-1, clone ASM-1, Chemicon®, from mouse
生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
ASM-1, monoclonal
物種活性
chicken, mouse, horse, rat, human, bovine
製造商／商標名
Chemicon®
技術
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG2a
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
human ... ACTA2(59)
一般說明
ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. This antibody reacts with many types of smooth muscle cells, such as those present in vascular walls, intestinal muscularis mucosae and propria, myometrium, stroma of various tissues, and is also positive for myoepithelial cells of various glands, notably salivary and mammary gland. Myogenic soft tissues detected include leiomyosarcomas, leiomyomas, and certain stromal cells surrounding infiltrating ductal carcinoma of the breast.
特異性
Expected to cross-react with bovine, equine and chicken. Reactivity with other species has not been confirmed.
This antibody is specific for the alpha-smooth-muscle isoform of actin (MW 43 kDa).
免疫原
Epitope: N-terminus
Synthetic peptide corresponding to the ten N-terminal amino acids of the alpha-smooth muscle isoform of actin.
應用
Anti-Actin Antibody, smooth muscle, clone ASM-1 detects level of Actin & has been published & validated for use in IF, IH, IH(P) & WB.
Immunohistochemistry:
1:2000 dilution of a previous lot was used on frozen and formalin-fixed, paraffin-embedded tissues; protease pretreatment is recommended for paraffin-embedded sections.
Immunofluorescence:
A previous lot of this antibody was used on Immunofluoresence.
Optimal working dilutions must be determined by the end user.
1:2000 dilution of a previous lot was used on frozen and formalin-fixed, paraffin-embedded tissues; protease pretreatment is recommended for paraffin-embedded sections.
Immunofluorescence:
A previous lot of this antibody was used on Immunofluoresence.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
品質
Evaluated by Western Blot on HUVEC lysates.
Western Blot Analysis:
1:1000 dilution of this antibody detected ACTIN, SM MUSC on 10 μg of HUVEC lysates.
Western Blot Analysis:
1:1000 dilution of this antibody detected ACTIN, SM MUSC on 10 μg of HUVEC lysates.
標靶描述
43 kDa
聯結
Replaces: 04-1040
外觀
Protein A purified
Format: Purified
Purified mouse monoclonal IgG2a presented as lyophilized material. Reconstitute in 1 mL of of distilled water.
Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide.
Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide.
儲存和穩定性
Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析報告
Control
Positive reaction using stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts.
Positive reaction using stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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訊號詞
Warning
危險聲明
危險分類
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
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