ABS989
Anti-E6AP Antibody
serum, from rabbit
别名:
Ubiquitin-protein ligase E3A, E6AP ubiquitin-protein ligase, Human papillomavirus E6-associated protein, Oncogenic protein-associated protein E6-AP, Renal carcinoma antigen NY-REN-54
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所有图片(2)
About This Item
分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41
推荐产品
生物源
rabbit
品質等級
抗體表格
serum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
human, mouse, rat
技術
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... UBE3A(7337)
一般說明
E6AP/UB3A is an interesting E3 ubiquitin protein ligase with newly discovered alternative functions as well. It is the founding member of the HECT (Homologous to E6AP C terminus) family of ubiquitin ligases and recent research is shedding new light on its influence in health and disease. Ubiquitin substrates for E6AP/UB3A ligase include RAD23A/B, MCM7 (items involved in DNA replication), Annexin A1, PML tumor suppressor and the cell cycle regulator CDKN1B. E6AP also catalyzes the high risk human papilloma virus E6 mediated ubiquitination of p53, which contributes to the neoplastic progression of cells infected by these viruses. E6AP has also been identified as a steroid hormone receptor transcriptional coactivator, and E6AP has a role in numerous diseases including cancers and neurological syndromes. Additionally, E6AP/UB3A is developmentally important for adipogenesis, granulocyte maturation and synaptic processes, where it acts as a down-regulator. IE6AP/UBE3A protein is active as a trimer of E6AP subunits and widely expressed.
免疫原
a small portion of the full length protein expressed as a recombinant
應用
Research Category
Signaling
Signaling
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
This Anti-E6AP Antibody is validated for use in Western Blotting and Immunoprecipitation for the detection of E6AP.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected E6AP in 10 µg of A431, C2C12, L6, mouse brain, and PC3 cell lysate.
Western Blotting Analysis: A representative lot detected E6AP in HeLa cell lysate (Talis, A. L., et al. (1998). JBC. 273:6439-6445).
Immunoprecipitation Analysis: A representative lot immunoprecipitated E6AP in HeLa cell lysate (Wynn, H. K., et al. (2000). Journal of Virology. 74(14):6408-6417).
Western Blotting Analysis: A representative lot detected E6AP in HeLa cell lysate (Talis, A. L., et al. (1998). JBC. 273:6439-6445).
Immunoprecipitation Analysis: A representative lot immunoprecipitated E6AP in HeLa cell lysate (Wynn, H. K., et al. (2000). Journal of Virology. 74(14):6408-6417).
品質
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected E6AP in 10 µg of A431 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected E6AP in 10 µg of A431 cell lysate.
標靶描述
~100 kDa observed. Uncharacterized band(s) may appear in some lysates.
外觀
Unpurified
Rabbit polyclonal serum with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
The role of E6AP in the regulation of p53 protein levels in human papillomavirus (HPV)-positive and HPV-negative cells.
Talis, A L, et al.
The Journal of Biological Chemistry, 273, 6439-6445 (1998)
W H Kao et al.
Journal of virology, 74(14), 6408-6417 (2000-06-23)
The E6 protein of the high-risk human papillomaviruses (HPVs) and the cellular ubiquitin-protein ligase E6AP form a complex which causes the ubiquitination and degradation of p53. We show here that HPV16 E6 promotes the ubiquitination and degradation of E6AP itself.
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