ABS310

Anti-hCE-1 Antibody

serum, from rabbit

• 别名: Carboxylesterase 1, Liver carboxylesterase 1, Acyl-coenzyme A:cholesterol acyltransferase, ACAT, Brain carboxylesterase hBr1, CE-1, hCE-1, Cocaine carboxylesterase, Egasyn, HMSE, Methylumbelliferyl-acetate deacetylase 1, Monocyte/macrophage serine estera
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: serum
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: human
• 技術: western blot: suitable
• NCBI登錄號: NP_001020365.1
• UniProt登錄號: P23141
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... CES1(1066)

说明

一般說明

Carboxylesterase 1 (hCE-1) is also known as Liver carboxylesterase 1, Acyl-coenzyme A:cholesterol acyltransferase, (ACAT), Cocaine carboxylesterase, Methylumbelliferyl-acetate deacetylase 1, Monocyte/macrophage serine esterase, Retinyl ester hydrolase, (REH), Serine esterase 1, and Triacylglycerol hydrolase (TGH). hCE-1 is involved in: the detoxification of xenobiotics and the activation of ester and amide prodrugs. hCE-1 functions to hydrolize the methyl ester group of cocaine to form benzoylecgonine, catalyze the transesterification of cocaine to form cocaethylene and the ethyl esterification of oleic acid to ethyloleate, and is activated by CHAPS. hCE-1 is expressed mostly in liver with lower levels found in the heart and lung.

特異性

This antibody does not cross react with human CE-2 (Prof. P. Potter, St. Jude Children′s Research Hospital, Memphis).

免疫原

Linear peptide corresponding to human hCE-1.

應用

Detect Carboxylesterase 1 using this rabbit polyclonal antibody, Anti-hCE-1 Antibody validated for use in western blotting.

Research Category
Signaling

Research Sub Category
Developmental Signaling

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected hCE-1 in 10 µg of human liver tissue lysate.
Western Blotting Analysis: A representative lot from an independent laboratory detected hCE-1 in hCE-1 transfected U373MG cell lysates, and demonstrated a loss of signal in non-transfected and hCE-2 transfected U373MG cell lysates (Prof. P. Potter, St. Jude Children′s Research Hospital, Memphis.).

品質

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected hCE-1 in 10 µg of HepG2 cell lysate.

標靶描述

~58 kDa observed. A homodimeric trimer of three hCE-1 molecules may be observed at ~190 kDa in some cell lysates (Prof. P. Potter, St. Jude Children′s Research Hospital, Memphis.).

外觀

Unpurified

Rabbit polyclonal serum with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析報告

Control
HepG2 cell lysate

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1