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Key Documents

ABE413

Sigma-Aldrich

抗NF-E2相关因子2抗体

from rabbit, purified by affinity chromatography

别名:

Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2, HEBP1, Nuclear factor erythroid derived 2-like 2

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

human

物種活性(以同源性預測)

mouse (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), rat (based on 100% sequence homology)

包裝

antibody small pack of 25 μg

技術

immunocytochemistry: suitable
western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

ambient

儲存溫度

2-8°C

目標翻譯後修改

unmodified

基因資訊

human ... NFE2L2(4780)

一般說明

NF-E2相关因子2(核因子红系2相关因子2)是一种转录激活因子,可与目标基因启动子区域的抗氧化反应元件(ARE)结合。NF-E2相关因子2可介导基因对氧化应激的协同上调。NF-E2相关因子2也可能参与β-珠蛋白簇基因的转录激活。NF-E2相关因子2广泛表达,在成年肌肉、肾脏、肺、肝和胎儿肌肉中表达量最高。

特異性

该抗体可识别NF-E2相关因子2的C末端

免疫原

与人NF-E2相关因子2 C端相对应的KLH偶联肽。
表位:C末端

應用

抗NF-E2相关因子2抗体是一种高度特异性的兔多克隆抗体,靶向NF-E2相关因子2 & 已通过蛋白质印迹和&ICC测试。
研究子类别
转录因子
研究类别
表观遗传学&核功能
蛋白印迹分析: 1 µg/mL代表性批次产品在10 µg Jurkat细胞裂解液中检测到NF-E2相关因子2。

免疫细胞化学分析:一个代表性批次产品以1:200的稀释度,在未经处理和过氧化氢处理的细胞中检测到NF-E2相关因子2(注意:细胞质染色在氧化应激下增加。)

品質

通过蛋白质印迹对Ramos细胞裂解液进行了评估。

蛋白质印迹分析:1 µg/mL该抗体在10 µg Ramos细胞裂解液中检测到NF-E2相关因子2。

標靶描述

观测值~75 kDa。已知存在68 kDa(异构体1)和66 kDa(异构体2)的两个异构体。由于在Nrf2中发现了大量酸性残基,这导致其在SDS-PAGE中发生异常迁移,因此在〜75 kDa处观察到该蛋白质(Abu-Bakar, A., et al. (2007) Drug Metab Dispos.35(5):787-794.)。NF-E2相关因子2的泛素化形式可在〜110 kDa处观察到(Li, J., et al. (2005).Toxicol Sci. 83(2):313-328.)。

外觀

亲和纯化
纯化的兔单克隆抗体,溶于含有0.1 M Tris-甘氨酸(pH 7.4)、150 mM NaCl和0.05%叠氮化钠的缓冲液中。

儲存和穩定性

自发运之日起,在 2-8°C 条件下可稳定保存1年

分析報告

对照
Ramos细胞裂解液

其他說明

浓度:请参考批次特异性浓缩物的分析证书。

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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