推荐产品
生物源
goat
品質等級
共軛
biotin conjugate
抗體表格
affinity purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
bovine, human
製造商／商標名
Chemicon®
技術
ELISA: suitable
dot blot: suitable
immunohistochemistry: suitable
western blot: suitable
適合性
not suitable for immunohistochemistry (Paraffin)
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... COL1A1(1277)
特異性
Human type I collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types II, III, IV, V, and VI. May show reactivity to type I collagen from other species. AB758B has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).
免疫原
Human placental collagen type I
應用
Anti-Collagen Type I Antibody, biotin conjugated is an antibody against Collagen Type Id for use in DB, ELISA, WB, IH.
Dot and slot blotting: 1:500-1:1,000
ELISA: 1:1,000-4,000
Indirect immunohistochemistry (frozen sections only): 1:20-1:40
The non-biotinylated version (Cat. # AB758) reacts with human collagen I in reduced westerns with supernatant samples from serum-free cultures of human fibroblasts , and has been cited for use in WB applications (PMID: 20089870)
Not recommended for paraffin tissue sections.
Optimal working dilutions must be determined by the end user.
ELISA: 1:1,000-4,000
Indirect immunohistochemistry (frozen sections only): 1:20-1:40
The non-biotinylated version (Cat. # AB758) reacts with human collagen I in reduced westerns with supernatant samples from serum-free cultures of human fibroblasts , and has been cited for use in WB applications (PMID: 20089870)
Not recommended for paraffin tissue sections.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
外觀
Affinity purified immunoglobulin, biotin labeled. Prior to purification the antisera was adsorbed against collagen type II, III, IV, V and VI immobilized on Sepharose™ 4B.. Liquid in phosphate buffered saline, pH 7.4, containing 0.1% sodium azide.
儲存和穩定性
Maintain at 2-8°C in undiluted aliquots for up to 12 months.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Increased expression of TSH receptor by fibrocytes in thyroid-associated ophthalmopathy leads to chemokine production.
Erin F Gillespie,Konstantinos I Papageorgiou,Roshini Fernando,Nupur Raychaudhuri et al.
The Journal of clinical endocrinology and metabolism null
Beneficial Regulation of Fibrillar Collagens, Heat Shock Protein-47, Elastin Fiber Components, Transforming Growth Factor-?1, Vascular Endothelial Growth Factor and Oxidative Stress Effects by Copper in Dermal Fibroblasts.
Neena Philips,Philips Samuel,Harit Parakandi,Sesha Gopal,Halyna Siomyk,Abraham Ministro et al.
Connective tissue research null
Stephen J Atkins et al.
Endocrinology, 156(12), 4731-4740 (2015-09-22)
A relationship between the actions of TSH and IGF-1 was first recognized several decades ago. The close physical and functional associations between their respective receptors (TSHR and IGF-1R) has been described more recently in thyroid epithelium and human orbital fibroblasts
Interleukin-6 production in CD40-engaged fibrocytes in thyroid-associated ophthalmopathy: involvement of Akt and NF-?B.
Gillespie, EF; Raychaudhuri, N; Papageorgiou, KI; Atkins, SJ; Lu, Y; Charara, LK; Mester et al.
Investigative Ophthalmology & Visual Science null
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