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Key Documents

AB5972

Sigma-Aldrich

Anti-Dishevelled-2 Antibody

Chemicon®, from rabbit

别名:

DVL2, DSH Homolog 2

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

purified immunoglobulin (Partially purified)

抗體產品種類

primary antibodies

無性繁殖

polyclonal

物種活性

human, mouse

製造商/商標名

Chemicon®

技術

immunohistochemistry: suitable
western blot: suitable

輸入

sample type hematopoietic stem cell(s)
sample type neural stem cell(s)

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... DVL2(1856)
mouse ... Dvl2(13543)

一般說明

DVL2 is a member of multigene family that also contains DVL1 and DVL3. The vertebrate dishevelled proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. DVL2 encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein.

特異性

Disheveiled-2 protein (dvl-2). By Western blot the antibody shows no cross reactivity with recombinant disheveiled-1 (dvl-1) or disheveiled-3 (dvl-3) protein. Pre-adsorption of the antibody with the immunogen peptide completely abolishes its reactivity by Western blot against disheveiled-2 protein.

免疫原

Synthetic peptide corresponding to amino acids 600-614 of human and mouse disheveiled-2 protein.

應用

Detect Dishevelled-2 using this Anti-Dishevelled-2 Antibody validated for use in WB, IH.
Research Category
Stem Cell Research
Research Sub Category
Hematopoietic Stem Cells

Neural Stem Cells
Western blot: 1:1,000 using ECL. The antibody reacts with a protein of ~95kDa. Faint reactivity was seen in the dvl-1 and dvl-3 HEK293 cell lysates suggesting there may be endogenous dvl-2 present.

Immunohistochemistry: 1:1,000 using the avidin-biotin system.

Dilute antibody in 50 mM PBS, pH 7.4 containing 1.5% sodium chloride and 1% normal animal serum (same species as the host of the secondary antibody).

Optimal working dilutions must be determined by end user.

標靶描述

80 kDa

外觀

Format: Purified
Partially purified immunoglobulin. Liquid in PBS, pH 7.4, containing 0.01M sodium azide.

儲存和穩定性

Maintain at -20°C in undiluted aliquots (not less than 10 μL) for up to 6 months. Avoid repeated freeze/thaw cycles. The use of high quality antiserum-grade plastic or glass is recommended.

分析報告

Control
SK-N-SH (human neuroblastoma) whole cell lysates, NIH 3T3 whole cell lysate, BT-20 (human mammary gland) cell lysate, FP (mouse embryonal carcinoma

testicular teratoma) cell lysate

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Glycogen synthase kinase-3beta immunoreactivity is reduced in the prefrontal cortex in schizophrenia.
Beasley, C, et al.
Neuroscience Letters, 302, 117-120 (2001)
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