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Merck
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Key Documents

AB5612

Sigma-Aldrich

Anti-Citrulline Antibody

serum, Chemicon®

别名:

CCP

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

serum

抗體產品種類

primary antibodies

無性繁殖

polyclonal

物種活性(以同源性預測)

mammals

製造商/商標名

Chemicon®

技術

ELISA: suitable
immunohistochemistry: suitable

適合性

not suitable for immunohistochemistry (Paraffin)

運輸包裝

wet ice

目標翻譯後修改

unmodified

特異性

Citrulline in glutaraldehyde fixed tissues or cells.



The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:



Compound Cross-reactivity ratio

Citrulline-G-BSA 1

Arginine-G-BSA 1/>100,000

Glutamine-G-BSA 1/>100,000

Ornithine-G-BSA 1/>100,000



Abbreviations:



(G) Glutaraldehyde

(BSA) Bovine Serum Albumin

免疫原

Citrulline-Glutaraldehyde-BSA

應用

Anti-Citrulline Antibody is a Rabbit Polyclonal Antibody for the detection of Citrulline & has been validated in ELISA & IHC.
Immunohistochemistry: 1:1,000-1:2,500 glutaraldehyde required for reactivity. (0.5%-2.5% is typical; fluorescence detection is not recommended. Blocking is with 3% normal serum with 0.1-0.2% triton X-100 usually. Primary antibody is diluted only in TBS or PBS with out blocking or triton X-100. Incubations are usually 3 hours 37C or 4C overnight. Detection is by ABC or PAP for best results. Not recommended for paraffin embedded tissues.

ELISA: 1:2500-1:5000

Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology

外觀

Rabbit antiserum. Liquid with 0.05% sodium azide

儲存和穩定性

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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The periodontium of periodontitis patients contains citrullinated proteins which may play a role in ACPA (anti-citrullinated protein antibody) formation.
Willem Nesse,Johanna Westra,Jacqueline E van der Wal,Frank Abbas,Anthony P Nicholas et al.
Journal of Clinical Periodontology null
Nitric oxide modulates peristaltic muscle activity associated with fluid circulation in the sea pansy Renilla koellikeri.
Anctil, M; Poulain, I; Pelletier, C
The Journal of Experimental Biology null
Charlotte Thålin et al.
Journal of thrombosis and haemostasis : JTH, 18(10), 2732-2743 (2020-07-13)
Recent data propose a diagnostic and prognostic capacity for citrullinated histone H3 (H3Cit), a marker of neutrophil extracellular traps (NETs), in pathologic conditions such as cancer and thrombosis. However, current research is hampered by lack of standardized assays. We aimed

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