推荐产品
生物源
rabbit
品質等級
抗體表格
serum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
human, monkey, mouse
應無反應活性
bovine
製造商／商標名
Chemicon®
技術
immunoprecipitation (IP): suitable
western blot: suitable
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
human ... APP(351)
特異性
Amyloid Precursor Protein (APP), Universal. Specific to amino acids 99-126.
免疫原
APP (aa 99-126) conjugated to KLH.
Epitope: universal
應用
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
This Anti-Amyloid Precursor Protein Antibody, universal is validated for use in IP, WB for the detection of Amyloid Precursor Protein.
Western blot: 1:250 - 1:1,000: need to reduce and alkylate disulfide bonds of APP to expose the epitope on APP transferred to nitrocellulose or PVDF prior to immunoblotting.
To reduce incubate the slide (Blot) in TBS (PBS) + 50mM 2-mercaptoethanol at 37°C for 3 h in the dark. Wash twice with TBS, then incubate in TBS (PBS) + 20 mg/ ml of Iodoacetic acid for 30 min at room temperature. Wash twice with TBS (PBS), block and process as usual.
Immunoprecipitation: Need to reduce and alkylate disulfide bonds to expose the epitope
Optimal working dilutions must be determined by end user.
To reduce incubate the slide (Blot) in TBS (PBS) + 50mM 2-mercaptoethanol at 37°C for 3 h in the dark. Wash twice with TBS, then incubate in TBS (PBS) + 20 mg/ ml of Iodoacetic acid for 30 min at room temperature. Wash twice with TBS (PBS), block and process as usual.
Immunoprecipitation: Need to reduce and alkylate disulfide bonds to expose the epitope
Optimal working dilutions must be determined by end user.
外觀
Serum. Liquid in PBS with 0.01% azide.
儲存和穩定性
Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Zhi-Xing Yao et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 16(12), 1677-1679 (2002-09-19)
Amyloid beta-peptide (Abeta), Abeta precursor protein (APP), apolipoprotein E (apoE), and elevated cholesterol levels have been linked to Alzheimer's disease (AD) pathology. High cholesterol levels increase APP and apoE expression in human NT2 neuron progenitor cells. A cholesterol-rich environment also
E Campbell et al.
Journal of neuroscience methods, 93(2), 133-138 (2000-01-14)
A novel polyclonal antibody (Ab993), specific for a KPI domain epitope of APP, was characterised for use in immunoprecipitation, Western blotting and immunohistochemistry. Conditioned medium from NTera2/D1 cells was used for immunoprecipitation and Western blots. Paraffin-embedded human brain sections were
Fuyan Chen et al.
Experimental and therapeutic medicine, 20(5), 88-88 (2020-09-26)
Alzheimer's disease (AD), the leading cause of age-related dementia, is characterized by abnormal β-amyloid accumulation. During learning, memory formation and consolidation, increased levels of histone H3 and H4 acetylation are observed. The present study reported significantly decreased level of H4K16ac
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