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一般說明
Optimized reagents for ultrasensitive β-gal assays
The BetaFluor β-Gal Assay Kit is designed for rapid, sensitive measurement of β-galactosidase activity in cell extracts. The kit features Reportasol Extraction Buffer, which enables efficient passive extraction of cell protein while maintaining optimal enzyme activity, and is configured for 96-well format. Because no mechanical operations are necessary for extraction, the assay is highly amenable to high-throughput reporter gene expression analysis. The BetaFluor Assay is designed for fluorometric detection with 500-fg sensitivity.
成分
•100 mgBetaFluor Substrate
•25 mlReportasol Extraction Buffer
•100 mlReaction Buffer
•50 mlStop Buffer
•1.6 ml1 M DTT
•25 mlReportasol Extraction Buffer
•100 mlReaction Buffer
•50 mlStop Buffer
•1.6 ml1 M DTT
警告
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
法律資訊
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
訊號詞
Danger
危險聲明
危險分類
Eye Dam. 1 - Skin Irrit. 2
儲存類別代碼
10 - Combustible liquids
Justine M Pinskey et al.
The Journal of biological chemistry, 292(37), 15192-15204 (2017-07-02)
Hedgehog (HH) signaling critically regulates embryonic and postnatal development as well as adult tissue homeostasis, and its perturbation can lead to developmental disorders, birth defects, and cancers. Neuropilins (NRPs), which have well-defined roles in Semaphorin and VEGF signaling, positively regulate
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