70608

pETBlue-1 DNA - Novagen

Novagen′s pETBlue-1 vector is designed to identify recombinants by traditional blue/white screening while also allowing T7lac promoter based expression of target genes.

• 分類程式碼代碼: 41106610
• NACRES: NA.51

属性

• 品質等級: 100
• 形狀: liquid
• 製造商／商標名: Novagen^®
• 儲存條件: OK to freeze; avoid repeated freeze/thaw cycles
• 運輸包裝: wet ice
• 儲存溫度: −20°C

说明

一般說明

The pETBlue-1 vector is designed to identify recombinants by traditional blue/white screening while also allowing T7lac promoter based expression of target genes. Screening is independent of expression because the T7lac expression promoter is in an opposed orientation relative to the E. coli promoter that mediates blue/white screening. pETBlue-1 facilitates the expression of native unfused proteins and allows convenient subcloning of target genes already fused to existing detection and purification tags. The EcoR V cloning site is appropriately spaced downstream of an E. coli ribosome binding site. Inserts must encode an ATG start codon at their 5′ end if expression is desired. The sequence is numbered from the first base of the T7 promoter sequence.

警告

Toxicity: Standard Handling (A)

法律資訊

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable