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product name
BLR(DE3)感受态细胞- Novagen, BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences.
生物源
Escherichia coli
品質等級
製造商/商標名
Novagen®
儲存條件
OK to freeze
avoid repeated freeze/thaw cycles
增長模式
adherent or suspension
形態學
rod shaped
技術
microbiological culture: suitable
細胞轉化
transformation efficiency: >2×106 cfu/μg
運輸包裝
dry ice
儲存溫度
−70°C
一般說明
基因型:F-ompT hsdSB(rB- mB-) gal lac ile dcm Δ(srl-recA)i306::Tn10 (tetR)(DE3)
本产品含有转基因生物(GMO)。在欧盟范围内,转基因物种分别受欧洲议会和理事会的2001/18 /EC和2009/41/EC指令及其成员国中国家所实施规范的约束。该法规规定我们有义务索取您以及处理转基因生物的机构的相关信息。请点击here获得最终用户声明(EUD)表格。
本产品含有转基因生物(GMO)。在欧盟范围内,转基因物种分别受欧洲议会和理事会的2001/18 /EC和2009/41/EC指令及其成员国中国家所实施规范的约束。该法规规定我们有义务索取您以及处理转基因生物的机构的相关信息。请点击here获得最终用户声明(EUD)表格。
BLR是BL21的recA-衍生物,可提高质粒单体产量,并有助于稳定含有重复序列,或其产物可能导致DE3原噬菌体丢失的靶质粒(1,2)。
DE3表明宿主是λDE3的溶酶原,因此携带一个由lacUV5启动子控制的T7 RNA聚合酶基因的染色体拷贝。这些菌株适合于通过IPTG诱导从pET载体中克隆的靶基因生产蛋白质。
DE3表明宿主是λDE3的溶酶原,因此携带一个由lacUV5启动子控制的T7 RNA聚合酶基因的染色体拷贝。这些菌株适合于通过IPTG诱导从pET载体中克隆的靶基因生产蛋白质。
BLR是BL21的recA衍生物,可提高质粒单体产量,并有助于稳定含有重复序列的靶质粒。
成分
0.4 ml1 ml成分
•2 × 0.2 ml5 × 0.2 mlBLR(DE3)感受态细胞
•2 × 2 ml4 × 2 mlSOC培养基
•2 ng2 ng测试质粒
•2 × 0.2 ml5 × 0.2 mlBLR(DE3)感受态细胞
•2 × 2 ml4 × 2 mlSOC培养基
•2 ng2 ng测试质粒
警告
毒性:多个毒性值,请参阅MSDS(O)
其他說明
1.A. Roca (U. of Wisconsin), personal
communication.
2.Studier, F. W. (1991) J. Mol.Biol. 219,
37-44.
communication.
2.Studier, F. W. (1991) J. Mol.Biol. 219,
37-44.
法律資訊
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 2
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