654205-M

TNF-α，人，重组，大肠埃希菌

• 别名: TNF-α，人，重组，大肠埃希菌, rhTNF-α，rh-恶液质素，肿瘤坏死因子-α
• CAS号: 94948-59-1
• MDL號碼: MFCD00148692
• 分類程式碼代碼: 12352202
• NACRES: NA.27

属性

• 生物源: human
• 品質等級: 100
• 重組細胞: expressed in E. coli
• 化驗: ≥95% (SDS-PAGE)
• 形狀: lyophilized
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze
• 雜質: ≤0.1 ng/μg Endotoxin (ng/μg TNF-α)
• 溶解度: distilled water: 0.1—1 mg/mL
• 儲存溫度: −70°C
• 基因資訊: human ... TNF(7124)

说明

一般說明

Research area: Apoptosis

Tumor Necrosis Factor alpha (TNF α), a homotrimer protein made of 157 amino acids, is produced by macrophages/monocytes, T-lymphocytes, and natural killer cells during acute inflammation. soluble and transmembrane form. The precursor form of TNF-α is in transmembrane form (tmTNF-α), which is processed to release soluble form (sTNF-α).

應用

TNF-α, Human, Recombinant, E. coli has been used to stimulate NF-κB activation and study the effects of pancreatic elastase on cellular responses.

生化／生理作用

Recombinant, human tumor necrosis factor-α expressed in E. coli., a cytokine produced primarily by activated macrophages, activates a variety of immune defense mechanisms by interactions with polymorphonuclear leukocytes, T cells, antibody-producing B lymphocytes, fibroblasts, and hematopoietic bone marrow cells. Activity is not species-specific. Induces apoptosis in human blood and bone marrow neutrophils and in endothelial cells. Increases the iNOS levels in vascular smooth muscle cells. Involved in pathophysiological processes of several chronic and acute diseases. Stimulates stress-activated protein kinase (SAPK). ED₅₀ = 20-50 pg/ml as measured in a cytotoxicity assay with the TNF-α-susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)

Tumor Necrosis Factor alpha (TNF alpha), is an inflammatory cytokine. It coordinates a range of signaling events within cells, leading to necrosis or apoptosis. TNF-α initiates signal transduction pathways by binding to TNFR-1 and TNFR-2. These pathways cause cellular processes such as cell survival, differentiation, and proliferation.

警告

毒性：标准处理（A）

重構

复溶后，等分并冷冻用于长期保存（-20°C or -70°C），或冷藏（4°C）短期保存。避免溶液反复冻融。贮备溶液在-20°C或-70°C下可稳定保存至多3个月，在4°C下可稳定至多1个月。

用无菌dH₂O进行复溶，最终浓度为0.1-1 mg/ml。应在低内毒素培养基或含有载体蛋白（如热灭活FCS或组织培养级BSA）的水性缓冲液中，进行进一步稀释。

其他說明

Merck Index 13, 9882.
Tsuchida, H., et al. 1995.J. Immunol. 154, 2403.
Westwick, J.K., et al. 1995.J. Biol. Chem. 270, 22689.
Polunovsky, V.A., et al. 1994.Exp.Cell Res.214, 584.
Koide, M., et al. 1993.FEBS Lett.318, 213.
Vilcek, J., and Lee, T.H.1991.J. Biol. Chem.266, 7313.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable