525295

PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20)

liquid, clone PY20, Calbiochem^®

• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: PY20, monoclonal
• 形狀: liquid
• 包含: ≤0.1% sodium azide as preservative
• 物種活性（以同源性預測）: all
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; avoid repeated freeze/thaw cycles
• 同型: IgG2b
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 目標翻譯後修改: phosphorylation (pTyr)
• 基因資訊: human ... PID1(55022)

说明

一般說明

Purified mouse monoclonal antibody. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states.

Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.

This PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20) is validated for use in ELISA, Immunoblotting, Frozen Sections, ICC, IP for the detection of Phosphotyrosine.

免疫原

KLH conjugated to phosphotyrosine, glycine, and alanine

應用

ELISA (see comments)

Immunoblotting (1:500-1:1000, see application references)

Frozen Sections (see application references)

Immunocytochemistry (see application references)

Immunoprecipitation (see comments)

包裝

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

外觀

In 10 mM sodium phosphate, pH 7.4.

重構

Following initial thaw, aliquot and freeze (-20°C).

分析報告

Positive Control
Any cell lysate or tissue extract

其他說明

Binding is inhibited by phosphotyrosine. Does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. This antibody has also been reported to work for ELISA and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Glenney, J.R., et al. 1988. J. Immunol. Methods109, 277.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable