251765

DAR-4M AM

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.

• 别名: DAR-4M AM, Diaminorhodamine-4M AM
• 经验公式（希尔记法）: C₂₈H₃₁IN₄O₅
• 分子量: 630.47
• 分類程式碼代碼: 12352202
• NACRES: NA.25

属性

• 品質等級: 100
• 化驗: ≥98% (HPLC)
• 形狀: solution
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; protect from light
• 顏色: dark red
• 螢光: λ_ex ~560 nm; λ_em ~575 nm
• 運輸包裝: ambient
• 儲存溫度: −20°C

说明

一般說明

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH above 4.0. Excitation max: ~560 nm; emission max: ~575 nm.

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of O₂, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH.

生化／生理作用

Cell permeable: yes

Primary Target
Nitric oxide (NO) fluorescent indicator

Product does not compete with ATP.

Reversible: no

包裝

Packaged under inert gas

警告

Toxicity: Irritant (B)

外觀

In DMSO.

重構

Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution, discard any unused portion.

其他說明

Kojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.

法律資訊

Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: 188.6 °F
• 閃點（°C）: 87 °C