208773

Calpain Substrate IV, Cell-Permeable, Fluorogenic

The non-permeant fluorogenic Calpain Substrate III is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage.

• 别名: Calpain Substrate IV, Cell-Permeable, Fluorogenic
• 经验公式（希尔记法）: C₁₂₇H₂₀₆N₅₀O₂₇S
• 分子量: 2897.38
• 分類程式碼代碼: 12352200
• NACRES: NA.77

属性

• 品質等級: 100
• 化驗: ≥95% (HPLC)
• 形狀: lyophilized solid
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; desiccated (hygroscopic); protect from light
• 溶解度: DMSO: 5 mg/mL
• 螢光: λ_ex 480 nm; λ_em 320 nm
• 運輸包裝: ambient
• 儲存溫度: −20°C

说明

一般說明

The non-permeant fluorogenic Calpain Substrate III (Cat. No. 208771) is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage. In addition to the expected improvement in permeability, the modified peptide also exhibits enhanced Calpain B sensitivity (k_cat = 0.2 s^-1, k_cat/K_M = 5000 M^-1 s^-1) when compared to the parental peptide (k_cat = 0.17 s^-1, k_cat/K_M = 680 M^-1 s^-1). Its suitability in detecting cellular Ca²⁺-dependent calpain activity is demonstrated both in intact Drosophila melanogaster S2 cells following ionomycin stimulation and in S2 lysates via direct Ca²⁺ addition.

The non-permeant fluorogenic Calpain Substrate III (Cat. No. 208771) is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage. In addition to the expected improvement in permeability, the modified peptide also exhibits enhanced Calpain B sensitivity (k_cat = 0.2 s^-1, k_cat/K_M = 5000 M^-1 s^-1) when compared to the parental peptide (k_cat = 0.17 s^-1, k_cat/K_M = 680 M^-1 s^-1). Its suitability in detecting cellular Ca²⁺-dependent calpain activity is demonstrated both in intact Drosophila melanogaster S2 cells following ionomycin stimulation and in S2 lysates via direct Ca²⁺ addition.

The non-permeant fluorogenic Calpain Substrate III is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage.

包裝

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

序列

(DABCYL)-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-Glu(EDANS)-Arg-Arg-Arg-Arg-Arg-Arg-Arg-NH₂

外觀

Supplied as a trifluoroacetate salt

重構

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

其他說明

Banoczi, Z., et al. 2008. Bioconjug. Chem.19, 1375.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable