208731

Calpain Substrate II, Fluorogenic

Cyclic fluorogenic substrate for the quantitative determination of calpain -1 and -2.

• 别名: Calpain Substrate II, Fluorogenic, Suc-LY-AMC
• 经验公式（希尔记法）: C₂₉H₃₁N₃O₇
• CAS号: 94367-20-1
• 分子量: 533.57
• MDL號碼: MFCD00057900
• 分類程式碼代碼: 12352200
• NACRES: NA.77

属性

• 品質等級: 100
• 化驗: ≥95% (TLC)
• 形狀: solid
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; desiccated; protect from light
• 顏色: off-white
• 溶解度: DMSO: soluble
• 螢光: λ_ex ~380 nm; λ_em ~460 nm
• 運輸包裝: ambient
• 儲存溫度: −20°C
• InChI: 1S/C29H33N3O8/c1-4-16(2)27(32-24(34)11-12-25(35)36)29(39)31-22(14-18-5-8-20(33)9-6-18)28(38)30-19-7-10-21-17(3)13-26(37)40-23(21)15-19/h5-10,13,15-16,22,27,33H,4,11-12,14H2,1-3H3,(H,30,38)(H,31,39)(H,32,34)(H,35,36)/t16?,22?,27-/m0/s1
• InChI 密鑰: VSWNAJAOJZXWBM-FTJGSZSVSA-N

说明

一般說明

Cyclic fluorogenic substrate for the quantitative determination of calpain -1 and -2. Also suitable for measuring the peptidase activity of the 20S proteasome. Typical concentrations range from 10 - 100 µM, depending on assay conditions.

Fluorogenic substrate for the quantitative determination of porcine calpain I and II activity. Also serves as a substrate for other proteases including proteasome. (excitation wavelength: 380 nm; emission wavelength: 460 nm)

生化／生理作用

Cell permeable: no

Primary Target
Calpain -1 and -2

Product does not compete with ATP.

Reversible: no

警告

Toxicity: Standard Handling (A)

序列

Suc-Leu-Tyr-AMC

重構

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

其他說明

Seol, J.H., et al. 1989. FEBS Lett. 247, 197.
Woo, K.M., et al. 1989. J. Biol. Chem. 264, 2088.
Sasaki, T., et al. 1984. J. Biol. Chem.259, 12489.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable