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品質等級
製造商／商標名
Upstate®
技術
activity assay: suitable
affinity binding assay: suitable
UniProt登錄號
檢測方法
chemiluminescent
運輸包裝
wet ice
基因資訊
human ... CDC42(998)
包裝
Kit capacity: 30 assays
成分
Rac/cdc42 Assay Reagent (PAK1 PBD, agarose) (Cat.# 14-325)
Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
100X GTPγS, 10mM (Cat.# 20-176)
100X GDP, 100mM (Cat.# 20-177)
Anti-cdc42
Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
100X GTPγS, 10mM (Cat.# 20-176)
100X GDP, 100mM (Cat.# 20-177)
Anti-cdc42
品質
Routinely evaluated by immunoprecipitating cdc42-GTP from 3T3/NIH cell lysate that has been loaded in vitro with GTPγS. Subsequent detection in western immunoblot using anti-cdc42.
聯結
Replaces: SGT430
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
訊號詞
Danger
危險聲明
危險分類
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
儲存類別代碼
10 - Combustible liquids
Isao Usui et al.
The Journal of biological chemistry, 278(16), 13765-13774 (2003-02-05)
We investigated the role of cdc42, a Rho GTPase family member, in insulin-induced glucose transport in 3T3-L1 adipocytes. Microinjection of anti-cdc42 antibody or cdc42 siRNA led to decreased insulin-induced and constitutively active G(q) (CA-G(q); Q209L)-induced GLUT4 translocation. Adenovirus-mediated expression of
Distinct mechanisms of clathrin-independent endocytosis have unique sphingolipid requirements.
Zhi-Jie Cheng, Raman Deep Singh, Deepak K Sharma, Eileen L Holicky, Kentaro Hanada et al.
Molecular Biology of the Cell null
V Benard et al.
The Journal of biological chemistry, 274(19), 13198-13204 (1999-05-01)
A major function of Rac2 in neutrophils is the regulation of oxidant production important in bacterial killing. Rac and the related GTPase Cdc42 also regulate the dynamics of the actin cytoskeleton, necessary for leukocyte chemotaxis and phagocytosis of microorganisms. Although
S J Taylor et al.
Current biology : CB, 6(12), 1621-1627 (1996-12-01)
Ras proteins play an essential role in the transduction of signals from a wide range of cell-surface receptors to the nucleus. These signals may promote cellular proliferation or differentiation, depending on the cell background. It is well established that Ras
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