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等級
RIA grade
化驗
≥98% (agarose gel electrophoresis)
形狀
solid
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
雜質
≤3.0 ash
≤5.0% moisture
顏色
off-white
溶解度
0.15 M sodium chloride: 10 mg/mL
正離子痕跡
heavy metals: ≤10 ppm
運輸包裝
ambient
儲存溫度
2-8°C
一般說明
牛血清白蛋白(BSA)是一种α-螺旋状的非糖基化球状蛋白，含有17个二硫键。
牛血清白蛋白，组分V(RIA和ELISA级)是可用作稳定剂和载体蛋白的纯化血浆蛋白。在非变性条件下通过改良Cohn方法和辛酸热激处理制备。在150 mM NaCl中10%的pH值为6.8-7.4。
應用
牛血清白蛋白，组分V(RIA和ELISA级)已用于：
- 在免疫组化(IHC)分析中用于胎盘组织切片的封闭
- 作为Krebs缓冲液组分，用于体外葡萄糖刺激胰岛素分泌(GSIS)实验和药物/棕榈酸制备
- 作为溶剂对照孵育小鼠全血，用于研究体内血小板聚集
生化／生理作用
牛血清白蛋白(BSA)是细胞培养基的重要成分，可运输脂肪酸、激素和药物。它是酶联免疫吸附试验(ELISA)中常用的封闭剂。
警告
毒性：标准处理（A）
重構
在复溶后，分装并冷冻保存（-20°C）。储备溶液在-20°C下可稳定保存至多6个月。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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The continuous search for new molecules with therapeutic abilities has led to the synthesis and characterization of a large number of metal complexes, proven to exhibit potential as pharmacological agents through their antibacterial, antiviral, antifungal and antineoplastic properties. As serum
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Journal of immunological methods, 384(1-2), 148-151 (2012-06-27)
The enzyme-linked immunosorbent assay (ELISA) is an extremely common and powerful laboratory technique for detecting proteins by antibodies. Researchers frequently use bovine serum albumin (BSA) as a blocking agent to prevent non-specific binding of antigens and antibodies to the microtiter
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