1.25090

RoboColumn Fractogel SO3 (M)，0.2 ml

RoboColumn Fractogel^® SO3 (M), volume 0.2 mL, prepacked column

• 别名: Fractogel^® EMD SO₃ ⁻ (M) 树脂
• 分類程式碼代碼: 41115711

属性

• ligand: (Sulfoisobutyl)
• 品質等級: 100
• 材料: polypropylene housing
• 產品線: Fractogel^®
• 形狀: prepacked column
• 參數: ≤8 bar pressure; ~200 cm/hr max. flow rate
• 柱內徑 × 長度: 5 mm × 10 mm
• 容積: 0.2 mL
• 基質活性組: Methacrylate
• 平均粒徑: 40-90 μm
• 容量: ~130 mL, resin binding capacity
• 應用: mAb; recombinant protein; vaccine development
• 分離技術: cation exchange
• 儲存溫度: room temp

说明

一般說明

5 x 10 mm

特點和優勢

RoboColumn Fractogel^® SO3 (M), 0.2 ml enables:

• Increased binding strength at low pH
• High aggregate removal efficiency combined with high dynamic binding capacity
• Lower elution volumes for the highest purity levels
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel^® EMD SO₃^- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

法律資訊

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany