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Merck
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Key Documents

1.25072

Millipore

MiniChrom Column Fractogel SO3 (M), 5 ml

MiniChrom Column Fractogel® SO3 (M), volume 5 mL, prepacked column

别名:

Fractogel® EMD SO3 ⁻ (M) 树脂

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About This Item

分類程式碼代碼:
41115711

ligand

(Sulfoisobutyl)

材料

polypropylene housing

產品線

Fractogel®

形狀

prepacked column

參數

≤8 bar pressure
~200 cm/hr max. flow rate

柱內徑 × 長度

8 mm × 100 mm

容積

5 mL

基質活性組

Methacrylate

平均粒徑

40-90 μm

容量

~130 mL, resin binding capacity

應用

mAb
recombinant protein
vaccine development

分離技術

cation exchange

儲存溫度

15-25°C

一般說明

8 x 100mm

特點和優勢

MiniChrom Column Fractogel® SO3 (M), 5 ml enables:
  • Increased binding strength at low pH
  • High aggregate removal efficiency combined with high dynamic binding capacity
  • Lower elution volumes for the highest purity levels
  • Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution


Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel® EMD SO3- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).

The strength of the binding depends on the following:
  • the buffer system
  • pH value of the buffer which determines the surface charge of the protein
  • the concentration of the counter ions
  • the charge density on the support (protein binding capacity)

包裝

1 pkg Plastic bag(s)

法律資訊

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

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