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agency
FDA-BAM
ISO 16654
品質等級
描述
with Novobiocin (20 mg/L) acc. ISO 16654 GranuCult
無菌
non-sterile
形狀
granular
成份
Novobiocin, 20 mg/L
製造商／商標名
GranuCult® prime
技術
microbiological culture: suitable
pH值
7.1-7.5 (25 °C, 33 g/L in H2O, after autoclaving)
溶解度
33 g/L
應用
food and beverages
microbiology
pathogen testing
儲存溫度
15-25°C
適合性
Escherichia coli (O157:H7 )
selective for Escherichia coli (EHEC)
urea-degrading bacteria
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一般說明
Modified Tryptic Soy Broth with Novobiocin is recommended for the enrichment of enterohemorrhagic E. coli (EHEC). The nutrients in mTSB broth provide favorable growth conditions, while the mixture of bile salts No. 3 and Novobiocin suppress the growth of the Gram-positive microbial flora.
應用
mTSB broth with Novobiocin is used for the selective enrichment of enterohemorrhagic E. coli (EHEC) as well as detection of E. coli serotype O157:H7 in foods.
分析報告
Appearance (clearness): clear
Appearance (color): yellowish-brown
pH-value (25 °C): 7.2 - 7.6
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of E.coli and S.aureus
Additionaly monoculture of S.aureus
Inoculum on reference medium (Escherichia coli ATCC 35150 (O157)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 700728(WDCM 00014)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≥ 10000
Growth (Escherichia coli ATCC 35150 (O157)): >10 yellowish-brown colonies on CT-SMAC agar
Growth (Escherichia coli ATCC 700728 (WDCM 00014)): >10 yellowish-brown colonies on CT-SMAC agar
Growth Escherichia coliATCC 25922 (WDCM 00013): >10 pink colonies on CT-SMAC agar
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): total inhibition on TSA
Incubation:
mTSB broth: 18 - 24 hours at 41,5 °C, aerobic
Confirmation on CT-SMAC Agar resp. TSA: 24 ± 3 hours at 37 ± 1 °C aerobic
Appearance (color): yellowish-brown
pH-value (25 °C): 7.2 - 7.6
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of E.coli and S.aureus
Additionaly monoculture of S.aureus
Inoculum on reference medium (Escherichia coli ATCC 35150 (O157)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 700728(WDCM 00014)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≥ 10000
Growth (Escherichia coli ATCC 35150 (O157)): >10 yellowish-brown colonies on CT-SMAC agar
Growth (Escherichia coli ATCC 700728 (WDCM 00014)): >10 yellowish-brown colonies on CT-SMAC agar
Growth Escherichia coliATCC 25922 (WDCM 00013): >10 pink colonies on CT-SMAC agar
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): total inhibition on TSA
Incubation:
mTSB broth: 18 - 24 hours at 41,5 °C, aerobic
Confirmation on CT-SMAC Agar resp. TSA: 24 ± 3 hours at 37 ± 1 °C aerobic
腳註
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
法律資訊
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
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