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Key Documents

07-507

Sigma-Aldrich

抗磷酸化-BMK1/Erk5 (Thr218/Tyr220) 抗体

Upstate®, from rabbit

别名:

Anti-Anti-BMK1, Anti-Anti-ERK4, Anti-Anti-ERK5, Anti-Anti-PRKM7

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

affinity purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

rat, mouse, bovine, human

製造商/商標名

Upstate®

技術

inhibition assay: suitable (peptide)
western blot: suitable

同型

IgG

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

phosphorylation (pThr218/pTyr220)

基因資訊

bovine ... Mapk7(537703)
human ... MAPK7(5598)
mouse ... Mapk7(23939)
rat ... Mapk7(114509)

特異性

识别磷酸化-BMK1/Erk5(Thr218, Tyr220)。

免疫原

对应于人BMK1/Erk5氨基酸213-224的KLH偶联的合成肽

應用

研究子类别
MAP激酶
研究类别
信号传导
肽抑制分析:
用抗磷酸化BMK 1/ERK5(Thr218/Tyr220)(1µg/mL(1:1000)稀释)(目录号07-507)探测HeLa细胞裂解液,HeLa细胞裂解液+非磷酸化蛋白和HeLa细胞裂解液+磷酸化肽。使用与HRP偶联的驴抗兔IgG二抗和化学发光检测系统观察蛋白质。

箭头表示HeLa细胞裂解液&HeLa细胞裂解液+非磷酸化肽上的BMK1/ERK5(〜115 kDa)。HeLa细胞裂解液+磷酸化肽段上不存在条带,表明该抗体成功抑制了肽段。
该抗磷酸化-BMK1/Erk5(Thr218, Tyr220)抗体经过验证可用在WB中检测磷酸化-BMK1/Erk5(Thr218, Tyr220)。

品質

通过蛋白质印迹/肽抑制分析在HeLa细胞裂解液中进行了评估。

蛋白质印迹/肽抑制分析: 该抗体的1:1000稀释液在HeLa细胞裂解液中检测到磷酸化-BMK1/Erk5(Thr218/Tyr220)。

標靶描述

110-115kDa

外觀

亲和纯化的免疫球蛋白,溶于0.1 M Tris-甘氨酸(pH 7.4)、150 mM NaCl和0.05%叠氮化钠中。
免疫亲和纯化

儲存和穩定性

自收到之日起,在2-8°C可稳定保存1年。

分析報告

对照
使用山梨醇处理的HeLa细胞

其他說明

浓度:请参考批次特异性浓缩物的分析证书。

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1


分析证书(COA)

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访问文档库

Shan Jiang et al.
BioMed research international, 2017, 6895730-6895730 (2017-05-26)
Background. The aim of this study was to assess the effects of low concentrations of H2O2 on angiogenesis of human umbilical vein endothelial cells (HUVECs) in vitro and explore the underlying mechanisms. Methods. HUVECs were cultured and stimulated with different
Zeynep Madak-Erdogan et al.
Molecular cancer research : MCR, 12(5), 714-727 (2014-02-08)
Cancer cell motility and invasiveness are fundamental characteristics of the malignant phenotype and are regulated through diverse signaling networks involving kinases and transcription factors. This study establishes an estrogen receptor (ERα)/MAPK (ERK5)/cofilin (CFL1) network that specifies the degree of breast
G Zhou et al.
The Journal of biological chemistry, 270(21), 12665-12669 (1995-05-26)
We have identified two components of a new protein kinase signaling cascade, MAPK/ERK kinase 5 (MEK5) and extracellular signal-regulated kinase 5 (ERK5). The MEK5 cDNA was isolated by degenerate PCR and encodes a 444-amino acid protein, which has approximately 40%
Alessia Parascandolo et al.
Cell death & disease, 11(4), 289-289 (2020-04-29)
Opportunistic modification of the tumour microenvironment by cancer cells enhances tumour expansion and consequently eliminates tumour suppressor components. We studied the effect of fibroblasts on the circadian rhythm of growth and protein expression in colon cancer HCT116 cells and found
Kazuhiro Nakamura et al.
Methods in molecular biology (Clifton, N.J.), 661, 91-106 (2010-09-03)
Extracellular signal-regulated kinase 5 (ERK5) is also known as big MAPK (BMK1) or MAPK7. ERK5 is 115 kDa in mass and therefore larger than the other MAPKs such as ERK1/2, JNK, and p38. Like other MAPKs, ERK5 is ubiquitously expressed

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