07-502

Anti-p67-phox (mouse) Antibody

Upstate^®, from rabbit

• 别名: 67 kDa neutrophil oxidase factor, NADPH oxidase activator 2, Neutrophil NADPH oxidase factor 2, chronic granulomatous disease, autosomal 2, neutrophil cytosol factor 2, neutrophil cytosolic factor 2, neutrophil cytosolic factor 2 (65kD, chronic granuloma
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: mouse, rat
• 製造商／商標名: Upstate^®
• 技術: immunohistochemistry: suitable; western blot: suitable
• 同型: IgG
• NCBI登錄號: NP_000424
• UniProt登錄號: P19878
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Ncf2(17970) ; rat ... Ncf2(364018)

说明

一般說明

Activation of NADPH-oxidase dependent superoxide generation requires the participation of several cytosolic factors including p47phox, p67phox, p40phox and Rac (1 or 2). In the resting cell, p47phox, p67phox, and p40phox form a large molecular weight complex which can be recovered from the cytosolic fraction. Activation of the NADPH oxidase is initiated by the assembly of p47phox, p67phox, and Rac with cytochrome b558 in a 1:1:1:1 complex in the plasma membrane. p47phox, p67phox, and Rac exhibit cooperative binding, since increasing the concentration of any one component lowers the EC50 of the other components. A great deal of current research involves understanding the protein protein interactions, and how these change with the activation state.

特異性

Recognizes p67-phox, MW ~67 kDa.

免疫原

KLH-conjugated, synthetic peptides corresponding to the first 15 amino acids from the N-terminal and the last 15 amino acids on the C-terminal of mouse p67-phox.

應用

Detect p67-phox (mouse) using this Anti-p67-phox (mouse) Antibody validated for use in WB, IH.

Immunohistochemistry: This antibody has been demonstrated by an independent laboratory to detect p67-phox in male rat brain cortex tissue.

Research Category
Neuroscience

Research Sub Category
Oxidative Stress

品質

Evaluated by western blot in RIPA lysates from RAW 264.7 cells.

Western Blot Analysis: 0.5-2.0 µg/mL of this antibody detected p67-phox in RIPA lysates from RAW 264.7 cells.

標靶描述

67 kDa

外觀

Protein A purified

Format: Purified

Purified rabbit polyclonal IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
Rat brain tissue, RAW 264.7 cell lysate.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1