生物源
rabbit
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
pig, human, bovine, rabbit, mouse, canine, dog, rat
技術
ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable
同型
IgG
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
human ... ITPR1(3708)
一般說明
The InsP3 (inositol 1,4,5-trisphosphate) Receptor family are intracellular Ca2+ channels activated by the second messenger InsP3. There are at least three types of receptor, with distinct tissue-specific expression profiles and subcellular localizations; it is thought that the exact subtype profile conveys precise Ca2+ release responses. Release of Ca2+ from intracellular stores is the trigger for many cellular responses, including muscle contraction. Receptor activity is subject to feedback regulation by Ca2+ concentration, as high cytosolic Ca2+ concentrations shut down the channel. Ca2+ release is augmented by phosphorylation of the Receptor by PKA and PKG.
特異性
Reactivity with other species has not been determined.
Reacts with the C-terminal cytoplasmic domain of IP3 receptors Type 1, 2, and 3. IP3 receptors have been shown to play an important role in the regulation of Ca2+ release from internal storage sites during cell activation. AB1622 reacts with IP3 receptor (MW approx. 260 kDa) isolated from a variety of cell types (e.g. lymphocytes, macrophages, granulocytes, fibroblasts, epithelial, endothelial cells, skeletal muscle, cardiac muscle and brain tissues).
免疫原
Epitope: C-terminus
Synthetic peptide (KDSTEYTGPESYV) corresponding to the C-terminus of human IP3 Receptor type 1.
應用
Immunohistochemisty (paraffin): IP3 Receptor staining in rat cerebellum, tissue was pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1:100, using IHC-Select detection with HRP-DAB.
Immunofluorescence: 1:20 - 1:200 dilution from a previous lot. Light fixation, 2% PFA, 90% ethanol fixation; Visualization by confocal microscopy is required, as detection by standard fluorescent microscopy will not be adequate to detect the IP3R. Due to the intensity of confocal lasers, use of an anti-fading agent, such as DABCO, is strongly recommended.
ELISA: 1:2,000 dilution of a previous was used in ELISA.
Immunoprecipitation: 1:200 dilution of a previous lot was used in Immunoprecipitation.
Flow cytometry: 1:1,000 dilution of a previous lot was used in Flow Cytometry.
Radioimmunoassay: 1:10,000 dilution of a previous lot was used in Radioimmunoassay.
Optimal working dilutions must be determined by the end user.
Immunofluorescence: 1:20 - 1:200 dilution from a previous lot. Light fixation, 2% PFA, 90% ethanol fixation; Visualization by confocal microscopy is required, as detection by standard fluorescent microscopy will not be adequate to detect the IP3R. Due to the intensity of confocal lasers, use of an anti-fading agent, such as DABCO, is strongly recommended.
ELISA: 1:2,000 dilution of a previous was used in ELISA.
Immunoprecipitation: 1:200 dilution of a previous lot was used in Immunoprecipitation.
Flow cytometry: 1:1,000 dilution of a previous lot was used in Flow Cytometry.
Radioimmunoassay: 1:10,000 dilution of a previous lot was used in Radioimmunoassay.
Optimal working dilutions must be determined by the end user.
Research Category
Signaling
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
GPCR, cAMP/cGMP & Calcium Signaling
This Anti-IP3 Receptor Antibody is validated for use in WB, IF, IH(P), ELISA, IP, RIA for the detection of IP3 Receptor.
品質
Routinely evaluated by Western Blot on RAW 264 lysates.
Western Blot Analysis:
1:500-1,000 dilution of this lot detected IP3 Receptor on 10 μg of RAW 264 lysates.
Western Blot Analysis:
1:500-1,000 dilution of this lot detected IP3 Receptor on 10 μg of RAW 264 lysates.
標靶描述
~260 kDa
聯結
Replaces: AB1622
外觀
Purified
Format: Purified
Purified rabbit polyclonal IgG in buffer containing 10 mM sodium phosphate, 150 mM NaCl, and 0.01% sodium azide, pH 7.5.
儲存和穩定性
Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析報告
Control
RAW264 cell lysate
RAW264 cell lysate
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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