推荐产品
生物源
rabbit
抗體表格
serum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
human, eukaryotes, Tetrahymena sp.
製造商／商標名
Upstate®
技術
ChIP: suitable
immunocytochemistry: suitable
western blot: suitable
同型
IgG
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
acetylation (Penta-acetylation)
基因資訊
human ... H4C1(8359)
一般說明
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
特異性
Expected to cross-react with Tetrahymena and Eukaryote.
Other species not tested.
Recognizes hyperacetylated histone H4, Mr 10 kDa (SDS-PAGE); tri- and tetra-acetylated histone H4 on acid-urea gels. May cross-react with other acetylated proteins.
免疫原
KLH-conjugated, synthetic peptide
(AGGAcKGGAcKGGAcKGGAcKGGAcKGGC) related to amino acids 2 to 19 of Tetrahymena thermophila histone H4.
(AGGAcKGGAcKGGAcKGGAcKGGAcKGGC) related to amino acids 2 to 19 of Tetrahymena thermophila histone H4.
應用
Chromatin Immunoprecipitation:
Reported to immunoprecipitate transcriptionally active chromatin containing acetylated histone H4. (Perry, C.A., 1993)
Immunocytochemistry:
Reported to exclusively stain Tetrahymena macronuclei1 and chromatin in HeLa nuclei. (Perry, C.A., 1993)
Reported to immunoprecipitate transcriptionally active chromatin containing acetylated histone H4. (Perry, C.A., 1993)
Immunocytochemistry:
Reported to exclusively stain Tetrahymena macronuclei1 and chromatin in HeLa nuclei. (Perry, C.A., 1993)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-hyperacetylated Histone H4 (Penta) Antibody (Rabbit Polyclonal Antibody) validated in ChIP, ICC, WB to detect hyperacetylated Histone H4 (Penta) also known as H4 histone family member A.
品質
Routinely evaluated by Western Blot on HepG2 lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected HYPERACETYLATED HIS H4 on 10 μg of HepG2 lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected HYPERACETYLATED HIS H4 on 10 μg of HepG2 lysates.
標靶描述
Approx. 10 kDa
外觀
Unpurified
Rabbit polyclonal antiserum IgG in buffer containing 30% glycerol.
儲存和穩定性
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
分析報告
Control
Tetrahymena cells.
Tetrahymena cells.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Parental nucleosomes segregated to newly replicated chromatin are underacetylated relative to those assembled de novo.
Perry, C A, et al.
Biochemistry, 32, 13615-13623 (1993)
A network connecting Runx2, SATB2, and the miR-23a~27a~24-2 cluster regulates the osteoblast differentiation program.
Hassan, MQ; Gordon, JA; Beloti, MM; Croce, CM; van Wijnen, AJ; Stein, JL; Stein, GS; Lian, JB
Proceedings of the National Academy of Sciences of the USA null
The PB2 subunit of the influenza virus RNA polymerase affects virulence by interacting with MAVS and inhibiting IFN-{beta} expression.
Graef KM, Vreede FT, Lau YF, McCall AW, Carr SM, Subbarao K, Fodor E
Journal of virology null
Evidence for monomeric actin function in INO80 chromatin remodeling.
Kapoor, P; Chen, M; Winkler, DD; Luger, K; Shen, X
Nature Structural and Molecular Biology null
Cindy Law et al.
The Journal of biological chemistry, 290(21), 13234-13249 (2015-04-04)
H2A.Z is a histone H2A variant that is essential for viability in Tetrahymena and Drosophila and also during embryonic development of mice. Although implicated in diverse cellular processes, including transcriptional regulation, chromosome segregation, and heterochromatin formation, its essential function in
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