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Merck
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Key Documents

05-372

Sigma-Aldrich

Anti-GRB2 Antibody, clone 3F2

clone 3F2, Upstate®, from mouse

别名:

Anti-ASH, Anti-EGFRBP-GRB2, Anti-Grb3-3, Anti-MST084, Anti-MSTP084, Anti-NCKAP2

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

3F2, monoclonal

物種活性

bovine, mouse, hamster, rat, human

製造商/商標名

Upstate®

技術

immunohistochemistry: suitable
western blot: suitable

同型

IgG1κ

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... GRB2(2885)

特異性

N-terminal SH3 region of GRB2

免疫原

Recombinant GRB2/Ash

應用

This Anti-GRB2 Antibody, clone 3F2 is validated for use in IH, WB for the detection of GRB2.

品質

routinely evaluated in immunoblot on RIPA lysates of non-stimulated human A431 carcinoma cells, RIPA lysates of mouse 3T3/A31 fibroblasts, rat L6 myoblasts, Chinese hamster ovary cells or bovine brain extract

標靶描述

23-25kDa

外觀

Format: Purified
PBS containing 50% glycerol

分析報告

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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c-Cbl is transiently tyrosine-phosphorylated, ubiquitinated, and membrane-targeted following CSF-1 stimulation of macrophages
Wang, Y., et al
The Journal of Biological Chemistry, 271, 17-20 (1996)
A novel protein kinase C alpha-dependent signal to ERK1/2 activated by alphaVbeta3 integrin in osteoclasts and in Chinese hamster ovary (CHO) cells.
Rucci, N; DiGiacinto, C; Orru, L; Millimaggi, D; Baron, R; Teti, A
Journal of Cell Science null
Protein-tyrosine phosphatase 1D modulates its own state of tyrosine phosphorylation
Stein-Gerlach, M., et al
The Journal of Biological Chemistry, 270, 24635-24637 (1995)
M Rozakis-Adcock et al.
Nature, 363(6424), 83-85 (1993-05-06)
Many tyrosine kinases, including the receptors for hormones such as epidermal growth factor (EGF), nerve growth factor and insulin, transmit intracellular signals through Ras proteins. Ligand binding to such receptors stimulates Ras guanine-nucleotide-exchange activity and increases the level of GTP-bound
Y Chen et al.
The Journal of biological chemistry, 273(31), 19809-19816 (1998-07-25)
While it is well established that adenylyl cyclase and phospholipase C-beta are two proximal signal effectors for the calcitonin receptor, the more distal signaling pathways are less well characterized. G protein-coupled receptors can activate Erk1/2 by Gs-, Gi-, or Gq-dependent

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