推荐产品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
3F2, monoclonal
物種活性
bovine, mouse, hamster, rat, human
製造商/商標名
Upstate®
技術
immunohistochemistry: suitable
western blot: suitable
同型
IgG1κ
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... GRB2(2885)
特異性
N-terminal SH3 region of GRB2
免疫原
Recombinant GRB2/Ash
應用
This Anti-GRB2 Antibody, clone 3F2 is validated for use in IH, WB for the detection of GRB2.
品質
routinely evaluated in immunoblot on RIPA lysates of non-stimulated human A431 carcinoma cells, RIPA lysates of mouse 3T3/A31 fibroblasts, rat L6 myoblasts, Chinese hamster ovary cells or bovine brain extract
標靶描述
23-25kDa
外觀
Format: Purified
PBS containing 50% glycerol
分析報告
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
c-Cbl is transiently tyrosine-phosphorylated, ubiquitinated, and membrane-targeted following CSF-1 stimulation of macrophages
The Journal of Biological Chemistry, 271, 17-20 (1996)
A novel protein kinase C alpha-dependent signal to ERK1/2 activated by alphaVbeta3 integrin in osteoclasts and in Chinese hamster ovary (CHO) cells.
Journal of Cell Science null
Protein-tyrosine phosphatase 1D modulates its own state of tyrosine phosphorylation
The Journal of Biological Chemistry, 270, 24635-24637 (1995)
Nature, 363(6424), 83-85 (1993-05-06)
Many tyrosine kinases, including the receptors for hormones such as epidermal growth factor (EGF), nerve growth factor and insulin, transmit intracellular signals through Ras proteins. Ligand binding to such receptors stimulates Ras guanine-nucleotide-exchange activity and increases the level of GTP-bound
The Journal of biological chemistry, 273(31), 19809-19816 (1998-07-25)
While it is well established that adenylyl cyclase and phospholipase C-beta are two proximal signal effectors for the calcitonin receptor, the more distal signaling pathways are less well characterized. G protein-coupled receptors can activate Erk1/2 by Gs-, Gi-, or Gq-dependent
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