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Merck
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主要文件

05-361

Sigma-Aldrich

Anti-NFκB p52 Antibody

Upstate®, from mouse

别名:

DNA-binding factor KBF2, Lymphocyte translocation chromosome 10, Oncogene Lyt-10, nuclear factor of kappa light chain gene enhancer in B-cells 2, nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, nuclear factor of kappa light polypept

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

monoclonal

物種活性

human

製造商/商標名

Upstate®

技術

electrophoretic mobility shift assay: suitable
immunoprecipitation (IP): suitable
western blot: suitable

同型

IgG2a

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

目標翻譯後修改

unmodified

相关类别

一般說明

The NFκB transcription factor was originally identified as a protein complex consisting of a DNA binding subunit and an associated protein. The DNA binding subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFκB. It is synthesized as a protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFκB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells.

特異性

Does not cross-react with mouse or rat; other species cross-reactivity is unknown.
This antibody recognizes the p52 subunit of NFκB and its p100 precursor.

免疫原

GST-fusion protein corresponding to amino acids 1-444 of human NFκB p52 subunit (Schmidt, R., 1991;Bours, V., 1992).

應用

Anti-NFκB p52 Antibody is a Mouse Monoclonal Antibody for detection of NFκB p52 also known as DNA-binding factor KBF2, Lymphocyte translocation chromosome 10, Oncogene Lyt-10 & has been validated in EMSA, IP & WB.
Immunoprecipitation:
4 µg of a previous lot immunoprecipitated NFκB p52 and its p100 precursor from 500 µg of HeLa nuclear extract cell lysate.

Gel Shift Assay:
A previous lot of this antibody used 2 µg per gel shift assay (Bours, V., 1993).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

品質

Routinely evaluated by immunoblotting in HeLa nuclear extract or in human Raji cell, but not in mouse 3T3/A31 cell and rat PC 12 cell lysates

Western Blot Analysis:
0.5-2 µg/mL of this lot detected NFκB p52 and its p100 precursor in HeLa nuclear extract. A previous lot detected NFκB p52 and its p100 precursor in human Raji cell, but not in mouse 3T3/A31 cell and rat PC 12 cell lysates. Highly recommended for western blotting.

標靶描述

52 kDa; precursor at 100 kDa

外觀

Protein G Chromatography
Format: Purified
Purified mouse monoclonal IgG2a in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析報告

Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

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Mary Pulvino et al.
Blood, 120(8), 1668-1677 (2012-07-14)
Diffuse large B-cell lymphoma (DLBCL), the most common type of non-Hodgkin lymphoma, remains a partially curable disease. Genetic alterations affecting components of NF-κB signaling pathways occur frequently in DLBCL. Almost all activated B cell-like (ABC) DLBCL, which is the least
Alternative splicing variants of IkappaB beta establish differential NF-kappaB signal responsiveness in human cells
Hirano, F., et al
Molecular and cellular biology, 18, 2596-2607 (1998)
Tumor-derived p53 mutants induce NF-kappaB2 gene expression.
Scian, MJ; Stagliano, KE; Anderson, MA; Hassan, S; Bowman, M; Miles, MF; Deb, SP; Deb, S
Molecular and cellular biology null
vFLIP from KSHV inhibits anoikis of primary endothelial cells.
Efklidou, S; Bailey, R; Field, N; Noursadeghi, M; Collins, MK
Journal of Cell Science null
TNF Receptor-Associated Factor 1 is a Major Target of Soluble TWEAK.
Carmona Arana, JA; Seher, A; Neumann, M; Lang, I; Siegmund, D; Wajant, H
Frontiers in Immunology null

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