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Merck
所有图片(3)

文件

05-1471

Sigma-Aldrich

Anti-Rictor Antibody, clone 9F1.2

clone 9F1.2, from mouse

别名:

Rictor, AVO3, rapamycin-insensitive companion of mTOR, mAVO3

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

9F1.2, monoclonal

物種活性

human, mouse, rat

技術

immunoprecipitation (IP): suitable
western blot: suitable

同型

IgG2aκ

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... RICTOR(253260)

一般說明

Rictor, the rapamycin-insensitive complex, is part of the mTORC2 kinase complex which is responsible for phosphorylating the hydrophobic motif kinase of Akt/PKB. This complex also includes mTOR, mSin1 and mLST8/GL. mTORC2 is activated by growth factors and other mitogenic signals.

特異性

This antibody recognizes Rictor.

免疫原

Recombinant protein corresponding to human Rictor.

應用

Anti-Rictor Antibody, clone 9F1.2 detects level of Rictor & has been published & validated for use in WB & IP.
Immunoprecipitation:
Representative lot data.
1 ug/mL dilution of this antibody immunoprecipitated Rictor from 100 mg of HEK293 lysate.

品質

Evaluated by Western Blotting on 10 mg of HEK293 cell lysate.

標靶描述

190 kDa Observed

外觀

Format: Purified
Purified immunoglobulin in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

分析報告

Control
HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Rictor on 10 µg of HEK293 cell lysate.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Zheng Wang et al.
Neoplasia (New York, N.Y.), 23(11), 1147-1165 (2021-10-28)
Epithelial-mesenchymal transition (EMT) contributes to tumor invasion, metastasis and drug resistance. AKT activation is key in a number of cellular processes. While many positive regulators for either EMT or AKT activation have been reported, few negative regulators are established. Through
Sharon K Kuss-Duerkop et al.
PLoS pathogens, 13(9), e1006635-e1006635 (2017-09-28)
Influenza A virus usurps host signaling factors to regulate its replication. One example is mTOR, a cellular regulator of protein synthesis, growth and motility. While the role of mTORC1 in viral infection has been studied, the mechanisms that induce mTORC1
Mandy K Losiewicz et al.
Experimental eye research, 197, 108131-108131 (2020-07-06)
The retina is one of the most metabolically active tissues, yet the processes that control retinal metabolism remains poorly understood. The mTOR complex (mTORC) that drives protein and lipid biogenesis and autophagy has been studied extensively in regards to retinal
Mahefatiana Andrianifahanana et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 30(11), 3733-3744 (2016-11-03)
TGF-β plays a central role in the pathogenesis of fibroproliferative disorders. Defining the exact underlying molecular basis is therefore critical for the development of viable therapeutic strategies. Here, we show that expression of the facilitative glucose transporter 1 (GLUT1) is
Shan Wang et al.
Molecular and cellular biology, 35(7), 1299-1313 (2015-01-15)
Mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates a diverse array of cellular processes, including cell growth, survival, metabolism, and cytoskeleton dynamics. mTOR functions in two distinct complexes, mTORC1 and mTORC2, whose activities and substrate specificities are

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