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Merck
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主要文件

05-1352

Sigma-Aldrich

Anti-Histone H2B Antibody, clone 5HH2-2A8

ascites fluid, clone 5HH2-2A8, from mouse

别名:

H2B, Histone H2B, histone 2, H2be, histone cluster 2, H2be, H2B histone family, member Q

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

ascites fluid

抗體產品種類

primary antibodies

無性繁殖

5HH2-2A8, monoclonal

物種活性

mouse, human, rat

技術

immunocytochemistry: suitable
western blot: suitable

同型

IgG1κ

GenBank登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... H2BC21(8349)
mouse ... H2Bc21(319190)

一般說明

Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2B is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

特異性

Antibody recognizes Histone H2B near the C-terminus.

免疫原

Epitope: C-terminus
Ovalbumin-conjugated synthetic linear peptide corresponding to the C-terminus of Histone H2B.

應用

Anti-Histone H2B Antibody, clone 5HH2-2A8 is a Mouse Monoclonal Antibody for detection of Histone H2B also known as Histone H2B, histone 2 H2be & has been validated in WB, ICC.
Immunocytochemistry:
A 1:500 dilution of a representative lot of this antibody detected Histone H2B in A431, HeLa and NIH/3T3 cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

品質

Evaluated by western blot in HepG2 cell lysate.

Western Blot Analysis:
A 1:1,000-1:2,000 dilution of this antibody detected Histone H2B in 10 µg of HepG2 cell lysate.

標靶描述

~15 kDa

聯結

Replaces: MABE15

外觀

Unpurified
Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析報告

Control
HepG2 cell lysate, L6 cell lysate, Mouse Brain Lysate

法律資訊

GenBank is a registered trademark of United States Department of Health and Human Services

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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商品

Loading controls in western blotting application.

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