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一般說明
Paxillin is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and an SH3- and SH2-binding domain that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Tyrosine phosphorylation of paxillin occurs in response to growth factors, neuropeptides, and integrins. The major sites of tyrosine phosphorylation include Tyr31 and Tyr118. Both of these sites may be involved in Crk binding to paxillin during integrin-mediated cell adhesion. These sites may provide docking motifs for recruitment of other signaling molecules to focal adhesions.
特異性
The antibody detects Paxillin phosphorylated at Tyr31.
免疫原
Clone M102 was generated from phospho-Paxillin (Tyr31) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 31 of human paxillin. This human sequence is highly conserved in rat and mouse paxillin.
應用
Detect phospho-Paxillin (Tyr31) using this Anti-phospho-Paxillin (Tyr31) Antibody, clone M102 validated for use in WB.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
品質
Evaluated by western Blot in pervanadate treated L6 cell lysates.
Western Blot Analysis: 1:500 dilution of this lot detected phospho-Paxillin on 10 µg of pervanadate treated L6 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected phospho-Paxillin on 10 µg of pervanadate treated L6 lysates.
標靶描述
72 kDa
聯結
Replaces: MAB1146
外觀
Protein A chromatography
Format: Purified
Purified monoclonal IgG1 in phosphate-buffered saline, 50% glycerol, 1 mg/mL BSA, and 0.05% sodium azide.
儲存和穩定性
Maintain at -20°C for one year from date of receipt. Do not aliquot.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
分析報告
Control
Pervanadate treated L6 cell lysates
Pervanadate treated L6 cell lysates
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Krista A Riggs et al.
Journal of cell science, 125(Pt 16), 3827-3839 (2012-05-11)
Integrins are the primary receptors of cells adhering to the extracellular matrix, and play key roles in various cellular processes including migration, proliferation and survival. The expression and distribution of integrins at the cell surface is controlled by endocytosis and
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