04-767

Anti-CREB Antibody, clone NL904, rabbit monoclonal

culture supernatant, clone NL904, Upstate^®

• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: culture supernatant
• 抗體產品種類: primary antibodies
• 無性繁殖: NL904, monoclonal
• 物種活性: rat, mouse, human
• 製造商／商標名: Upstate^®
• 技術: immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG
• NCBI登錄號: NM_134442
• UniProt登錄號: P16220
• 運輸包裝: dry ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... CREB1(1385)

说明

一般說明

CREB is a beta ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2.

特異性

Broad species cross-reactivity expected due to sequence homology

Recognizes CREB.

免疫原

Epitope: a.a. 5-24

peptide (C-SGAENQQSGDAAVTEAENQQ) corresponding to amino acids 5-24 of human CREB

應用

Detect CREB also known as cAMP-response Element Binding Protein with Anti-CREB Antibody, clone NL904 (Rabbit Monoclonal Antibody), that has been demonstrated to work in IP & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

品質

Routinely evaluated by immunoblot on HeLa nuclear extract

標靶描述

Mr ~43 kDa

聯結

Replaces: 04-218

外觀

Cultured supernantant in 0.05% sodium azide

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. It is suggested product be sub-aliquoted to avoid repeated freeze / thaw cycles.

分析報告

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable