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Key Documents

03-0176-00

Millipore

SMC® Human IL-10 High Sensitivity Immunoassay Kit

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

描述

LLOQ range: 0.1-0.2 pg/mL, refer to CoA forlot-specific LLOQ

物種活性

rat, mouse, feline, canine, nonhuman primates

製造商/商標名

SMC®

技術

single molecule counting: suitable

assay range

inter-assay cv: <20%
intra-assay cv: <15%
(Intra-assay n=20; Inter-assay n=5)

儲存溫度

2-8°C

一般說明

The SMC® Human IFN-b1 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format.
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.

特異性

Species Reactivity
Although IL-10 was detectable in non-human primate, mouse, rat, canine, and feline plasma, the assay has not been validated for these species.

Accuracy:
  • Minimum of 5 serum samples spiked with average recovery of 87% with a range of 84-93%
  • Minimum of 5 plasma samples spiked with average recovery of 90% with a range of 82-97%

應用

The sensitivity of the Single Molecule Counting (SMC®) technology gives the SMC® Human IL-10 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-10 in human serum and plasma.

包裝

96 well assay

成分

  • Assay Buffer
  • IL-10 Coated Beads
  • Standard Diluent
  • IL-10 Detection Antibody
  • IL-10 Standard
  • 10X Wash Buffer
  • Buffer D
  • Elution Buffer B

法律資訊

SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

象形圖

Skull and crossbonesHealth hazard

訊號詞

Danger

危險分類

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


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See how you can enhance your immunology research through the power of high sensitivity immunoassays by harnessing Single Molecule Counting (SMC®) technology to precisely characterize interferons, interleukins, and immunoglobins.

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