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Key Documents

767972

Sigma-Aldrich

Cell Free Amino Acid Mixture-15N

5-100 mM in water, 98 atom % 15N

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About This Item

分類程式碼代碼:
12352209
NACRES:
NA.12

同位素純度

98 atom % 15N

形狀

solid

濃度

5-100 mM in water

技術

bio NMR: suitable
protein expression: suitable

運輸包裝

dry ice

儲存溫度

−20°C

應用

This product suitable as source of Stable Isotope Labeled amino acids for Cell Free Protein Synthesis. For Mass Spec applications, consider product 909653, Stable Isotope Labeled Amino Acid Mixture for Mass Spec.

The product may appear turbid, due to the relatively high concentration of tyrosine. Once diluted for use, solution should be clear.

包裝

1 mL aqueous solution of all 20 amino acids.
This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

分析報告

Amino acid concentrations vary by lot. Please refer to the specific lot Certificate of Analysis.

Approximate concentrations (mM):
ASP 60
THR 35
SER 35
GLU 40
PRO 20
GLY 100
ALA 100
VAL 40
MET 10
ILE 30
LEU 45
TYR 10
PHE 16
HIS 5
LYS 15
ARG 10
GLN 20
ASN 20
TRP 20
CYS 20

需要但未提供

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2 - Skin Irrit. 2

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

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Filippo Caschera et al.
BioTechniques, 58(1), 40-43 (2015-01-22)
Here we present a procedure for preparing amino acid mixtures--having both the desired composition and a physiological pH--at high concentrations for cell-free expression systems. Up to 2.1 mg/mL of active protein was synthesized in batch mode reactions with an all

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