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58970-U

Supelco

SUPELCOSIL™ LC-18-T (3 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

Synonym(s):

LC-18-T HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

SUPELCOSIL™ LC-18-T HPLC Column, 3 μm particle size, L × I.D. 15 cm × 4.6 mm

material

stainless steel column

Agency

suitable for USP L1

product line

SUPELCOSIL™

feature

endcapped

manufacturer/tradename

SUPELCOSIL™

packaging

1 ea of

extent of labeling

12.3% carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

15 cm × 4.6 mm

surface area

170 m2/g

surface coverage

surface coverage 3.1 μmol/m2

matrix

silica gel, spherical particle platform
fully porous particle

matrix active group

C18 (octadecyl) phase

particle size

3 μm

pore size

120 Å

operating pH

2-7.5

application(s)

food and beverages

separation technique

reversed phase

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General description

SUPELCOSIL LC-18-T columns feature an octadecylsilane bonded phase and a special surface treatment for efficient separations of nucleotides. Each batch of packing material is tested to ensure good peak shape for a representative nucleotide, adenosine diphosphate (ADP). Chromatography of other compounds that exhibit metal chelating properties also can be improved by using this phase.

Application

  • A new validated HPLC method for the determination of quercetin: Application to study pharmacokinetics in rats.: This study utilizes the SUPELCOSIL LC-18-T HPLC Column to develop a new HPLC method for the determination of quercetin, significantly enhancing the ability to study its pharmacokinetics in biological samples. The research not only improves our understanding of quercetin′s therapeutic effects but also aids in optimizing its dosage for better clinical outcomes. The robust method ensures accurate and reproducible results, critical for advancing pharmacological research and supporting health sciences (Abdelkawy et al., 2017).

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
282437-06
282437-05
282437-04
282437-03
282437-02

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Mattia Quattrocelli et al.
JCI insight, 4(24) (2019-12-20)
In humans, chronic glucocorticoid use is associated with side effects like muscle wasting, obesity, and metabolic syndrome. Intermittent steroid dosing has been proposed in Duchenne Muscular Dystrophy patients to mitigate the side effects seen with daily steroid intake. We evaluated
Khaled S Abdelkawy et al.
Biomedical chromatography : BMC, 31(3) (2016-08-25)
A simple, accurate, and reproducible high-performance liquid chromatography (HPLC) method has been developed and validated for the quantification of quercetin (QR) in rat plasma. The method involves a simple protein precipitation procedure to extract both QR and thymoquinone (TQ), the

Protocols

HPLC Analysis of Adenosine Nucleosides on Supelcosil™ LC-18-T

Separation of Adenosine 5′-triphosphate disodium salt hydrate, BioXtra, ≥99% (HPLC), from microbial; Adenosine 5′-diphosphate sodium salt, bacterial, ≥95% (HPLC); 2′-Deoxyadenosine 5′-monophosphate, Sigma Grade, 98-100%

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