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Supelco

Ascentis® Express C8, 2 μm UHPLC Column

2 μm particle size, L × I.D. 3 cm × 3 mm

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

material

stainless steel column

Quality Level

100

Agency

suitable for USP L7

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

1000 bar max. pressure (14500 psi)
60 °C temp. range

technique(s)

LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

3 cm × 3 mm

surface area

120 m2/g

impurities

<5 ppm (metals)

matrix

Fused-Core particle platform
superficially porous particle

matrix active group

dimethyloctyl phase

particle size

2 μm

pore size

90 Å pore size

pH

2-9

application(s)

food and beverages

separation technique

reversed phase

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General description

Ascentis® Express 2μm C8 UHPLC columns are based on a 90 Å Fused-Core® particle design. The Fused-Core particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.4-micron thick porous shell and the small overall particle size of 2-microns. The densely bonded, extensively endcapped dimethyloctyl stationary phase of Ascentis Express 2μm C8 provides a stable, reversed-phase packing that can be used for basic, acidic, or neutral compounds.

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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Certificates of Analysis (COA)

Lot/Batch Number
USKYY001256
USKYY001255
USKYY001254
USKYY001253

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Ugandar Reddy Inugala et al.
Journal of chromatographic science, 51(5), 453-459 (2012-10-13)
This paper describes the development of a rapid, novel, stability-indicating gradient reversed-phase high-performance liquid chromatographic method and associated system suitability parameters for the analysis of naproxcinod in the presence of its related substances and degradents using a quality-by-design approach. All
Sreenivasa Rao Chitturi et al.
Journal of pharmaceutical and biomedical analysis, 55(1), 31-47 (2011-02-15)
This paper proposes a simple and selective RP-HPLC method for the determination of process impurities and degradation products (degradants) of atazanavir sulfate (ATV) drug substance. Chromatographic separation was achieved on Ascentis(®) Express C8, (150mm×4.6mm, 2.7μm) column thermostated at 30°C under
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 81-82, 126-132 (2013-05-07)
A closed-vessel microwave-assisted extraction (MAE) technique was optimized for the first time for the extraction of polyphenols from raw propolis. The results obtained by means of response surface experimental design methodology showed that the best global response was reached when
Monika A Olszewska
Journal of separation science, 35(17), 2174-2183 (2012-07-19)
An HPLC method of high resolution has been developed and validated for the simultaneous determination of ten prominent flavonoid aglycones in plant materials using a fused-core C18-silica column (Ascentis® Express, 4.6 mm × 150 mm, 2.7 μm). The separation was
Imran Ali et al.
Biomedical chromatography : BMC, 26(8), 1001-1008 (2012-01-13)
Superficially porous silica particles columns (SPSPCs) are manufactured by different companies. The most common have the brand names Halo, Ascentis Express and Kinetex. These columns provide super fast, sharp peaks and moderate sample loading and back pressure. These are available
View All Related Papers

Articles

Ascentis® Express C8 U/HPLC Columns

When a C18 doesn′t give the desired separation, or your sample contains compounds that are known to be difficult to retain or resolve on a C18, consider changing to an Ascentis® Express C8 column.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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