ZMS1035
Anti-MAG Antibody, clone 513 ZooMAb® Mouse Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Myelin-associated glycoprotein, Siglec-4a
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About This Item
UNSPSC Code:
12352203
Recommended Products
biological source
mouse
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
513, recombinant monoclonal
monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 69.07 kDa
observed mol wt ~90 kDa
species reactivity
rat, mouse
species reactivity (predicted by homology)
human, chicken, bovine, frog
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG1
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... MAG(4099)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Specificity
Clone 513 is a ZooMAb mouse recombinant monoclonal antibody that specifically detects Myelin-associated glycoprotein.
Immunogen
L2 epitope-carrying glycoproteins from 1- to 2- day-old chicken brains.
Application
Anti-MAG, clone 513 ZooMAb, Cat. No. ZMS1035, s a recombinant Mouse monoclonal antibody that targets Myelin Associated Glycoprotein and is tested in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected MAG in rat brain tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected MAG in rat brain tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected MAG in Oligodendrocyte precursor cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected MAG in rat brain tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected MAG in Oligodendrocyte precursor cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Myelin-associated glycoprotein (Siglec-4a, MAG) is encoded by the MAG (also known as GMA) gene (Gene ID: 4099) in chicken. MAG is a single-pass type I membrane protein that is expressed in myelin-forming cells when oligodendrites initiate contact with axons. It is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 20-516), a transmembrane domain (aa 517-536), and a cytoplasmic domain (aa 518-626). MAG is a member of the immunoglobulin superfamily that is found in both peripheral and CNS myelin, where it plays a role in the formation and maintenance of myelin sheath. It stabilizes contacts between myelinating axons and glial processes. It also participates in the formation of morphologically intact myelin sheath that results in periaxonal cytoplasmic collar of Schwann cells and compact myelin structure of oligodentrocytes. MAG is also considered to be important in maintenance of myelin with aging. MAG protects motoneurons against apoptosis and injury that is mediated via its interaction with neuronal reticulon receptor 4R (RTN4R) and Reticulon-4 receptor-like 2 protein (RTN4RL2). Three isoforms of MAG protein have been described that are produced by alternative splicing. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Poltorak, M., et al. (1987). J Cell Biol. 105(4); 1893-1899; Quarles, RH. (2007). J. Neurochem. 100(6); 1431-1448).
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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