SRP8050

Profilin from Toxoplasma gondii

recombinant, expressed in E. coli, >95% (SDS-PAGE)

• Synonym(s): Inflammatory profilin, T Cell Membrane Protein 1
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: Toxoplasma gondii
• recombinant: expressed in E. coli
• Assay: >95% (SDS-PAGE)
• form: lyophilized
• mol wt: ~20 kDa by SDS-PAGE
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: <0.01 EU/μg endotoxin, tested
• color: white
• UniProt accession no.: Q58NA1
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: Toxoplasma gondii ... PRF(7900190)

Description

Biochem/physiol Actions

Profilin is a protozoan parasite protein. It activates toll-like receptor 11 (TLR11), which is an intracellular receptor localized on the endoplasmic reticulum (ER). TLR11 acts as an innate immune sensor for T. gondii profilin (TgPRF).The 12 membrane-spanning ER-resident protein UNC93B1 directly binds TLR11 and control the activation of dendritic cells in response to profilin (Toxoplasma gondii) and parasitic infection in vivo. TLR12 also has an ability to interact with (TgPRF). TLR12 is adequate for recognizing profilin in plasmacytoid dendritic cells (pDCs), whereas TLR11 and TLR12 are both needed in macrophages and conventional DCs. In contrast to TLR11, TLR12-deficient mice fail to resist T. gondii infection.

Physical form

Lyophilized from PBS.

Reconstitution

Reconstitute with 100 μL deionized water.

Other Notes

Recombinant profilin (Toxoplasma gondii) (aa 1-163).

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable