Skip to Content
Merck
All Photos(1)

Key Documents

SRP3292

Sigma-Aldrich

R-SPONDIN-1 human

recombinant, expressed in CHO cells, ≥95% (SDS-PAGE), ≥95% (HPLC)

Synonym(s):

Rspo1

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in CHO cells

Assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

potency

1.0-3.0 μg/mL ED50

mol wt

26.0-40.0 kDa

packaging

pkg of 20 μg

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

Q2MKA7

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... RSPO1(284654)

General description

R-Spondin-1 (Rspo-1) belongs to the (Rspo) family of Wnt modulators. Currently, the (Rspo) family consists of four structurally related secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. Rspo-1 is expressed in certain areas of the developing central nervous system, as well as in adrenal glands, ovary, testis, thyroid, and trachea. Recombinant human R-Spondin-1 is a 26.7kDa protein consisting of 243 amino acid residues. Due to glycosylation, R-Spondin-1 migrates at an apparent molecular weight of approximately 40.0kDa by SDS PAGE analysis under reducing conditions.

Biochem/physiol Actions

Rspo (R-Spondin) can interact with the Frizzled/LRP6 (LDL receptor related protein 6) receptor complex in a manner that stimulates the Wnt/beta-catenin signaling pathway. Disruption of Rspo-1 gene causes recessive syndrome with XX sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Rspo-1 is also associated with radioresistance of tumor cells.

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

R-Spondin proteins: a novel link to beta-catenin activation.
Kim KA, et al.
Cell Cycle, 5, 23-26 (2006)
R-spondin1 is essential in sex determination, skin differentiation and malignancy.
Parma P, et al.
Nature Genetics, 38, 1304-1309 (2006)
Xuefeng Gu et al.
Oncotarget, 6(12), 9756-9765 (2015-04-14)
Although radiation therapy is the most effective postoperative adjuvant treatment, it does not substantially improve the long-term outcomes of glioma patients because of the characteristic radioresistance of glioma. We found that R-Spondin1 (Rspo1) expression was elevated in high-grade gliomas and
Minke E Binnerts et al.
Proceedings of the National Academy of Sciences of the United States of America, 104(37), 14700-14705 (2007-09-07)
The R-Spondin (RSpo) family of secreted proteins act as potent activators of the Wnt/beta-catenin signaling pathway. We have previously shown that RSpo proteins can induce proliferative effects on the gastrointestinal epithelium in mice. Here we provide a mechanism whereby RSpo1
Yong-Guo Zhang et al.
Autophagy, 15(11), 1935-1953 (2019-03-22)
Extracellular HMGB1 (high mobility group box 1) is considered as a damage-associated molecular pattern protein. However, little is known about its intracellular role. We studied the mechanism whereby intestinal epithelial HMGB1 contributes to host defense, using cell culture, colonoids, conditional

Articles

3D Organoid Culture: New In Vitro Models of Development and Disease

rganoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service