SRP0721
AKT1 (E17K), Active
PRECISIO® Kinase, recombinant
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About This Item
UNSPSC Code:
12352202
Recommended Products
recombinant
expressed in baculovirus infected Sf9 cells
Assay
70-95% (SDS-PAGE)
form
frozen liquid
specific activity
50-75 nmol/min·mg (radiometric)
mol wt
~85 kDa
concentration
0.1 μg/μL
accession no.
NM_005163
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... AKT1(207)
Application
Useful for kinase assay.
Biochem/physiol Actions
Recombinant full-length human AKT1 (E17K) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. AKT1/PKBalpha is a serine/threonine kinase that belongs to the AKT family. AKT1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). AKT1 is frequently overexpressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas and prostate as well as lymphomas and leukemias (2).
Reconstitution
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Storage and Stability
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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