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SCA20

Supelco

Sucrose Assay Kit

sufficient for 20 assays

Synonym(s):

Sucrose Test Kit

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About This Item

EC Number:
200-334-9
UNSPSC Code:
12164500
NACRES:
NA.84

usage

sufficient for 20 assays

Quality Level

100

analyte chemical class(es)

sugars (sucrose)

technique(s)

photometry: suitable

application(s)

food and beverages
general analytical

storage temp.

2-8°C

Related Categories

General description

Sucrose is a disaccharide, naturally occurring carbohydrate found in plants and a major product during photosynthesis.

Application

This kit is for the quantitative, enzymatic determination of sucrose in food and other materials. Sucrose is hydrolyzed to glucose and fructose by invertase. Glucose and fructose are phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to sucrose concentration.

Kit Components Only

Product No.
Description
  • glucose assay reagent 50 mL
  • sucrose assay reagent 1 mL/vial
  • sucrose standard 500 mg

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

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Bergmeyer, H.U. and Bernt, E.
Methods of Enzymatic Analysis, 1176-1176 (1974)
Ye Ji Lee et al.
Frontiers in plant science, 12, 644455-644455 (2021-07-20)
Nitrogen is one of the most important macro-nutrients for plant growth and crop productivity. The amount of synthetic nitrogen fertilizers supplied to crops has dramatically increased, leading to a notable rise in crop yields. However, excessive nitrogen use has an
Raja S Payyavula et al.
BMC plant biology, 12, 39-39 (2012-03-21)
Plant secondary metabolites, including phenylpropanoids and carotenoids, are stress inducible, have important roles in potato physiology and influence the nutritional value of potatoes. The type and magnitude of environmental effects on tuber phytonutrients is unclear, especially under modern agricultural management
Ching M Wai et al.
The Plant journal : for cell and molecular biology, 92(1), 19-30 (2017-07-04)
The altered carbon assimilation pathway of crassulacean acid metabolism (CAM) photosynthesis results in an up to 80% higher water-use efficiency than C3 photosynthesis in plants making it a potentially useful pathway for engineering crop plants with improved drought tolerance. Here
Quantitative 1H NMR with external standards: use in preparation of calibration solutions for algal toxins and other natural products.
Burton IW, Quilliam MA, Walter JA.
Analytical Chemistry, 77(10), 3123-3131 (2005)
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Protocols

Enzymatic Food Analysis

Enzymatic methods for food analysis are highly specific and offer considerable time and cost savings over other methods, especially from the sample preparation standpoint. We offer a wide variety of convenient kits and reagents for rapid and reliable enzymatic food analysis.

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