SAE0044

Choline Oxidase from Arthrobacter sp.

recombinant, expressed in E. coli ≥12 units/mg protein

• Synonym(s): COD, COX, ChOx, Choline: oxygen 1-oxidoreductase
• Enzyme Commission number: 1.1.3.17 (BRENDA, IUBMB)
• UNSPSC Code: 12352204

Properties

• biological source: bacterial (arthrobacter sp.)
• Quality Level: 200
• recombinant: expressed in E. coli ≥12 units/mg protein
• form: powder
• specific activity: ≥12 units/mg protein
• storage condition: (Tightly closed.Dry. Keep locked up or in an area accessible only to qualified or authorized; persons)
• technique(s): activity assay: suitable
• UniProt accession no.: A0A0Q9NRQ3; A0A0Q9PH20
• storage temp.: −20°C

Description

General description

Research area: Cell Signaling

Choline oxidase from Arthrobacter sp. is a flavoprotein, which is a member of the glucose–methanol–choline (GMC). The codA gene encodes the choline oxidase enzyme.

Application

Choline oxidase from Arthrobacter sp. has been used in the enzymatic determination of choline in milk using a flow injection analysis (FIA) system with potentiometric detection. It has been used for fluorescent detection of choline released during autotaxin (ATX) activity in conditioned media and plasma.

Biochem/physiol Actions

Choline oxidase from Arthrobacter sp. is a flavoprotein, which is a member of the GMC-oxidoreductase family. The enzyme catalyzes the four-electron-oxidation of choline to glycine betaine via the intermediate betaine aldehyde, in two sequential FAD-dependent reaction steps. The enzyme is useful for enzymatic determination of phosphatidylcholine by coupling with phospholipase D or determination of sphingomyelin by coupling with sphingomyelinase and for cholinesterase activity assays.Choline oxidase (COD) mediating glycine betaine biosynthetic pathway plays a vital role in creating stress resistant transgenic plants.

Unit Definition

One unit will form 1 μmole of H₂O₂ with oxidation of 1 μmole of choline to betaine aldehyde per min at pH 8.0 at 37 °C. Note: During the conversion of choline to betaine by choline oxidase, 2 μmoles of H₂O₂ are produced for every μmole of choline.

Preparation Note

It is recommended to reconstitute choline oxidase in phosphate buffer at pH 7.5 supplemented with 10mM EDTA. Reconstituted material can be stored at 2-8 °C for at least 2 weeks.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334 - H412
• Precautionary Statements: P261 - P273 - P284 - P304 + P340 + P312 - P501
• Hazard Classifications: Aquatic Chronic 3 - Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable