SAB3700041

Anti-Bovine IgM (μ-chain specific) antibody produced in rabbit

whole antiserum, lyophilized powder

• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: whole antiserum
• antibody product type: secondary antibodies
• clone: polyclonal
• form: lyophilized powder
• species reactivity: bovine
• technique(s): immunohistochemistry: suitable; indirect ELISA: suitable; western blot: suitable
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

During the B-cell development, IgM (immunoglobulin M) is the first expressed antibody. It is present as a monomer on the surface of naive B cells. Antigenic stimulation and upon maturation, the monomeric IgM units (190kDa), are linked by disulfide bonds to form a pentameric immunoglobulin structure. It also but rarely forms a hexameric structure. The pentameric IgM is linked by a disulfide bond, a polypeptide chain and a J-chain. The μ -chain consists of five domains- VH, Cμ1, Cμ2, Cμ3 and Cμ4.

Specificity

This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against Bovine IgM and Bovine Serum. No reaction was observed against Bovine IgG F(ab′)2 or Bovine IgG F(c).

Immunogen

Bovine IgM mu heavy chain

Biochem/physiol Actions

The J-chain of the multimeric IgM (immunoglobulin M) structure is responsible for secretion at the mucosal surfaces. The pentameric form of IgM, has a greater affinity towards antigens (with multiple repeating epitopes) compared to the monomers. IgM pentamer enables better opsonization of antigens for destruction and fixing complement.

Physical properties

Antibody format: Antiserum

Physical form

Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Reconstitution

Reconstitute with 1.0 mL deionized water (or equivalent).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable