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P3563

Sigma-Aldrich

Phosphate Buffered Saline

powder, pH 7.4, with 0.05% Tween® 20

Synonym(s):

1X PBS + 0.05% TWEEN® 20, 1X PBS + 0.05% TWEEN® 20, PBS-T, PBST

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About This Item

UNSPSC Code:
41105319
eCl@ss:
32129211
NACRES:
NA.25

product name

Phosphate Buffered Saline with 0.05% TWEEN® 20, pH 7.4,

form

powder

Quality Level

200

solubility

water: soluble, clear, colorless (1 pouch dissolved in 1L)

application(s)

cell analysis
diagnostic assay manufacturing

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General description

Phosphate Buffered Saline with Tween®-20, or PBST, is primarily used in immunoassays as a washing and blocking buffer. The phosphate buffer maintains a stable pH 7.4, suitable for many biological reactions and assays. The salt or saline solution in PBS-T provides the necessary ionic strength for optimal protein solubility and stability. The addition of 0.05% TWEEN®-20, a non-ionic detergent, helps reduce background signals by blocking non-specific binding sites on proteins. PBST buffer enhances the washing efficiency by removing unwanted substances and limits non-specific antibody binding sites, while preserving the integrity of the target proteins.

Application

Phosphate buffered saline has been used in:

  • western blotting
  • enzyme-linked immunosorbent assay (ELISA)
  • telomere-FISH (Tel-FISH) and telomere/immunostaining-FISH (Teli-Fish)
  • cell isolation

Packaging

Foil pouches

Reconstitution

Contents of one pouch dissolved in 1 L dH2O or ultrapure water to yield 0.01 M phosphate buffered saline, 0.138M NaCl, 0.0027M KCl, and 0.05% Tween 20, pH7.4.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Heaphy C M, et al.
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Telomere length assessment in human archival tissues: combined telomere fluorescence in situ hybridization and immunostaining
Meeker A K, et al.
The American Journal of Pathology, 160(4), 1259-1268 (2002)
The alternative lengthening of telomeres phenotype in breast carcinoma is associated with HER-2 overexpression
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