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H6291

Sigma-Aldrich

Interleukin-1β human

IL-1β, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture, endotoxin tested

Synonym(s):

IL-1β, Lymphocyte activating factor

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About This Item

MDL number:
MFCD00131399
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

human

Quality Level

100

recombinant

expressed in HEK 293 cells

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

potency

≤0.05 ng/mL EC50

quality

endotoxin tested

mol wt

dimer 18-25 kDa (glycosylated)

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<1 EU/μg

UniProt accession no.

P01584

storage temp.

−20°C

Gene Information

human ... IL1B(3553)

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Biochem/physiol Actions

Interleukin-1 (IL-1), originally known as Lymphocyte Activating Factor (LAF), activates T cell lymphocytes, which then proliferate and secrete interleukin-2 (IL-2). IL-1 is released primarily from stimulated macrophages and monocytes, but has also been shown to be released from several other types and is thought to play a key role in inflammatory and immune responses. Other synonyms for IL-1 include: endogenous pyrogen (EP), mitogenic protein (MP), Helper Peak-1 (HP-1), T Cell Replacing Factor III (TRF III or TRFM), B Cell Activating Factor (BAF) and B Cell Differentiation Factor (BDF) The two closely related agents Interleukin-1α (IL-1α) and Interleukin-1β (IL-1β) share 62% homology in amino acid sequence and elicit nearly identical biological responses. IL-1α and IL-1β are both approximately 17 kDa with some heterogeneity in the amount of glycosylation.

Physical form

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Preparation Note

HumanKine Recombinant Human IL-1β, expressed in human HEK 293 cells, is a glycosylated monomer with a molecular mass of 18-25 kDa. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note

The activity is determined by the dose-dependent stimulation of the proliferation of mouse D10S cells.

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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