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H4538

Sigma-Aldrich

Anti-Histone Deacetylase 5 (HDAC5) antibody, Mouse monoclonal

clone HDAC5-35, purified from hybridoma cell culture

Synonym(s):

Anti-HD5

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About This Item

MDL number:
MFCD04040361
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

HDAC5-35, monoclonal

form

buffered aqueous solution

mol wt

antigen ~124 kDa

species reactivity

rat, mouse, human

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 4-8 μg/mL using total cell extracts of NIH3T3 fibroblast cell or SW480 cell extract

isotype

IgG1

UniProt accession no.

Q9UQL6

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HDAC5(10014)
mouse ... Hdac5(15184)
rat ... Hdac5(84580)

General description

Monoclonal Anti-Histone Deacetylase 5 (HDAC5) (mouse IgG1 isotype) is derived from the HDAC5-35 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide. Mammalian HDACs can be divided into three classes according to sequence homology. Class II consists of the yeast Hda1-like proteins HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10. Histone deacetylase 5 (HDAC5) is a histone deacetylase, located on human chromosome 17q21.

Application

Anti-Histone Deacetylase 5 (HDAC5) antibody, Mouse monoclonal has been used in:
  • western blotting
  • immunostaining
  • enzyme-linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • immunoprecipitation

Biochem/physiol Actions

Histone deacetylase 5 (HDAC5) promotes progression of the cell cycle, cell proliferation and apoptosis. HDAC5 activity is important for the differentiation of muscle cells by binding, through its N-terminal domain, to the myocyte enhancer factor-2 (Mef2) protein, thus repressing expression of MEF2 downstream genes. Overexpression of HDAC5 in different cancer cells suppresses their growth by induction of apoptosis in a p53-independent manner. Reduced expression of the protein is observed in colon cancer and acute myeloid leukemia.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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HDAC5 promotes optic nerve regeneration by activating the mTOR pathway
Pita-TW, et al.
Experimental Neurology, 317, 271-283 (2019)
HDAC4 is required for inflammation-associated thermal hypersensitivity
Crow M, et al.
Faseb Journal (2015)
Megan Crow et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 29(8), 3370-3378 (2015-04-24)
Transcriptional alterations are characteristic of persistent pain states, but the key regulators remain elusive. HDAC4 is a transcriptional corepressor that has been linked to synaptic plasticity and neuronal excitability, mechanisms that may be involved in peripheral and central sensitization. Using
HDAC4 governs a transcriptional program essential for synaptic plasticity and memory
Sando III R, et al.
Cell, 151(4), 821-834 (2012)
Formononetin sensitizes glioma cells to doxorubicin through preventing EMT via inhibition of histone deacetylase 5.
Liu Q
International Journal of Clinical and Experimental Pathology, 8(6), 6434-6441 (2015)
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