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GE17-5478-01

Capto™ L

Cytiva 17-5478-01, pack of 25 mL

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

recombinant protein L (E. coli), mammalian free

packaging

pack of 25 mL

manufacturer/tradename

Cytiva 17-5478-01

storage condition

(20% Ehtanol)

matrix

rigid, highly cross-linked agarose

avg. part. size

85 μm (d₅₀v is the median particle size of the cumulative volume distribution)

working range

2-10(pH interval where the medium can be operated without significant change in function.)

suitability

suitable for bioprocess medium

storage temp.

2-8°C

General description

Capto™ L resin is designed for antibody fragment purification, when the fragment contains the kappa light chain.

Affinity resin for antibody fragment purification

Capto™ L combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which has a strong affinity to the variable region of antibody kappa light chains. The high binding capacity and affinity to a broad range of antibody fragments makes this resin an excellent capture platform for antibody fragments containing kappa light chains. Common uses include domain antibody (dAb), scFv, and Fab purification.

Using this protein L resin

Capto™ L resin requires minimal optimization and produces high purity in one separation step, resulting in significant savings during process development. This resin is also advantageous in manufacturing, because high selectivity of affinity capture reduces the number of subsequent polishing steps.

Application

Purification of antibody fragments using affinity chromatography

Features and Benefits

  • Suitable for the capture of a wide range of antibody fragments such as Fabs, dAbs, and single-chain fragment variable (scFv) .
  • High binding capacity, low ligand leakage, and selectivity for a broad range of antibody fragments reduces process time and amount of resin.
  • Enables a platform approach to antibody fragment purification.
  • Excellent productivity, process economy, and process design flexibility due to rigid matrix.
  • Hydrophilic base matrix ensures low levels of non-specific binding and low levels of host cell-derived impurities in the elution pool.
  • Fulfills industrial demands for security of supply, robust performance, and regulatory support.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Capto is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Performing a Purification of Antibody Fragments with Capto L Products

This page shows how to purify antibody fragments by affinity chromatography using Capto L products from GE Healthcare.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service